Specific Pathogen Free Baboon Research Resource (SPFBRR)

总体-项目摘要/摘要 无特定病原菌浣熊研究资源(SPFBRR)是唯一的国家级研究资源 美国国立卫生研究院受资助者可使用的SPF狒狒；联邦机构的内部研究计划，包括 FDA、NSF和NIH；以及生物医学研究的其他赞助商(私人基金会、制药 公司和合同研究机构)。在整个世界上绝对是独一无二的，世界上 SPFBRR有一份广泛的生物排除清单，列出了通常在其他野生和圈养动物中发现的19种病原体 非人灵长类动物群体。SPFBRR最初开发为R24，后来开发为P40，在 俄克拉荷马大学健康科学中心(OUHSC)与俄克拉荷马州立大学的合作 大学(OSU)。然而，最近一项决定结束了OUHSC的所有非人类灵长类研究， 有必要做出努力，重新安置SPFBRR。OUHSC领导层的全力努力导致 为这一宝贵而独特的国家研究资源寻找新的家园。米歇尔·E。 德克萨斯大学基林比较医学与研究中心(基林中心) 得克萨斯州巴斯特罗普的安德森癌症中心拥有满足以下需求的设施、专业知识、经验和基础设施 SPFBRR的当前和未来需求。通过这项拨款申请，我们建议将SPFBRR 到基林中心，通过从一个新的物种中分离出幼崽来加速SPF群体的生长 常规(非SPF)繁殖群体，并保持和扩大与研究项目的伙伴关系，使用 这些无价的SPF狒狒资源。在本申请所要求的支持期间， SPFBRR将扩大，以创建一个自给自足的SPF繁殖群体，其行为健康和 基因健壮的繁殖动物。这将消除对常规(非SPF)婴儿的需求 这将极大地降低将外来感染物引入SPFBRR的风险。 这个P40应用程序的总体目标是将SPFBRR重新定位到基林中心，以便 可以保存不可或缺的国家研究资源；为科学家提供教育和培训， 兽医、蜂群管理人员和动物护理员，从事与狒狒有关的工作；进行应用研究 改善资源；并支持需要研究项目设施和专业知识的研究人员 涉及SPF的狒狒。我们还将对主要组织相容性复合体进行mRNA测序 (MHC)SPFBRR中所有基础繁殖狒狒的I类等位基因；并且，我们将执行 对该资源中的26只动物进行基因组测序和系谱分析。此信息 将进一步提炼和增值SPFBRR提供的资源。设施最初设计用于 黑猩猩将被改变用途，以满足SPFBRR和基林其他研究设施的需求 该中心将提供空间，对SPF狒狒进行研究。

OVERALL – Project Summary/Abstract The Specific Pathogen Free Baboon Research Resource (SPFBRR) is the only national research resource of SPF baboons available to NIH grantees; intramural research programs of federal agencies, including the FDA, NSF and NIH; and other sponsors of biomedical research (private foundations, pharmaceutical companies, and contract research organizations). Absolutely unique in the entire world, the baboons in the SPFBRR have an extensive bioexclusion list of 19 pathogens normally found in other wild and captive nonhuman primate colonies. The SPFBRR was originally developed as an R24, and later as a P40, grant in a collaboration between the University of Oklahoma Health Sciences Center (OUHSC) and Oklahoma State University (OSU). However, a recent decision to end all nonhuman primate research at OUHSC has necessitated efforts to relocate the SPFBRR. An exhaustive effort by the leadership of OUHSC has resulted in the identification of a new home for this valuable and unique national research resource. The Michale E. Keeling Center for Comparative Medicine and Research (Keeling Center) of The University of Texas MD Anderson Cancer Center in Bastrop, Texas has the facilities, expertise, experience, and infrastructure to meet the current and future needs of the SPFBRR. With this grant application, we propose to relocate the SPFBRR to the Keeling Center, accelerate growth of the SPF colony through re-derivation of infant baboons from a conventional (non-SPF) breeding colony, and maintain and expand partnerships with research programs using these invaluable SPF baboon resources. During the period of support requested in this application, the SPFBRR will be expanded to create a self-sustaining SPF breeding colony of behaviorally healthy and genetically robust breeding animals. This will eliminate the need for infants from conventional (non-SPF) dams, and it will significantly decrease the risk of introducing adventitious infectious agents into the SPFBRR. The overall goals of this P40 application are to relocate the SPFBRR to the Keeling Center so that this indispensable national research resource can be preserved; provide education and training to scientists, veterinarians, colony managers, and animal caregivers who work with baboons; conduct applied research to improve the resource; and, support investigators who need facilities and expertise for research projects involving SPF baboons. We will also perform mRNA sequencing of the major histocompatability complex (MHC) class I alleles for all of the foundation breeding baboons in the SPFBRR; and, we will perform whole genome sequencing (WGS) on 26 animals in the resource for genomic and pedigree analysis. This information will further refine and add value to the resources provided by the SPFBRR. Facilities originally designed for chimpanzees will be repurposed to meet the needs of the SPFBRR and other research facilities at the Keeling Center will provide space to conduct research with SPF baboons.