Molecular Pathology Core

核心摘要/摘要 分子病理学核心为超级基金研究计划的研究人员提供 分子和形态学变化的评价所需的设备和技术专长， 细胞，组织和器官暴露于复杂的环境污染物后。国家核心住房- 最先进的设备，包括自动组织处理器，石蜡包埋中心，两个自动 切片机、低温恒温器、用于软组织切片的振动切片机、带投影的多头光学显微镜 能力，激光捕获显微切割系统，以及载玻片扫描仪， 形态结构的鉴定和定量。分子病理学核心由一名 主任，一名组织学家和一名组织技术员，他们在组织病理学和免疫细胞化学方面具有专长。 方法，包括固定、脱水、包埋、切片、组织学染色、免疫标记、高- 分辨率成像和定量图像分析。设施工作人员提供样品中的全包服务 准备，提供成像和图像分析方面的帮助，并为正在进行或未来的 研究项目。此外，核心为学生建立了广泛的培训计划， 使用中央设备的调查人员。 分子病理学核心的广泛，长期目标是有助于检测， 诊断和预防由暴露于复杂环境污染物引起的疾病。这么久- 我们将通过以下具体目标来实现我们的长期目标：具体目标1。提供高品质、低成本的 为超级基金研究计划的研究项目提供分子病理学方面的有效服务。 具体目标2。进一步整合分子病理学核心和Leduc生物成像中的方法 设施，其中房屋电子，荧光和共聚焦显微镜。具体目标3。识别 瓶颈的具体协议和设计创新的解决方案，以改善管道从建议 实验结果的公布。具体目标4。提供组织处理、切片、 组织学染色、免疫标记、高分辨率成像和定量图像分析。预期 这项工作的结果包括鉴定分子标志物和特定的病理变化， 暴露于复杂环境污染物后的细胞和组织形态。业绩及 开发的方法对超级基金研究中的各个研究项目至关重要 规划并促进环境污染物和混合物的循证风险评估。在 此外，在分子病理学核心培训的学生将配备跨学科的工具 以及解决环境科学未来问题的方法。

CORE SUMMARY/ABSTRACT The Molecular Pathology Core provides researchers in the Superfund Research Program with equipment and technical expertise necessary for the evaluation of molecular and morphological changes in cells, tissues, and organs following exposure to complex environmental contaminants. The Core houses state- of-the-art equipment, including an automated tissue processor, a paraffin embedding center, two automated microtomes, a cryostat, a vibratome for soft-tissue sectioning, a multiheaded light microscope with projection capabilities, a system for laser capture microdissection, and a slide scanner with analysis software for the identification and quantification of morphological structures. The Molecular Pathology Core is staffed by a director, a histologist and a histotechnician, who have expertise in histopathological and immunocytochemial methods, including fixation, dehydration, embedding, sectioning, histological staining, immunolabeling, high- resolution imaging, and quantitative image analysis. Facility staff provides all-inclusive services in sample preparation, offers assistance in imaging and image analysis and provides consultation for ongoing or future research projects. In addition, the core established an extensive training program for students and investigators who use the centrally available equipment. The broad, long-term objective of the Molecular Pathology Core is to contribute to the detection, diagnosis, and prevention of disease caused by exposures to complex environmental contaminants. This long- term goal will be achieved through the following specific aims: Specific Aim 1. Provide high-quality, cost- effective services in molecular pathology for the research projects in the Superfund Research Program. Specific Aim 2. Further integrate methodologies in the Molecular Pathology Core and the Leduc Bioimaging Facility, which houses electron, fluorescence, and confocal microscopes. Specific Aim 3. Identify the bottlenecks in specific protocols and design innovative solutions to improve the pipeline from the proposed experiments to publication of the results. Specific Aim 4. Provide training in tissue processing, sectioning, histological staining, immunolabeling, high-resolution imaging, and quantitative image analysis. The expected outcome from this work includes the identification of molecular markers and specific pathological changes in cell and tissue morphology following exposure to complex environmental contaminants. The results and the developed methodologies will be essential to the individual research projects in the Superfund Research Program and contribute to evidence-based risk assessment of environmental contaminants and mixtures. In addition, students who are trained in the Molecular Pathology Core will be equipped with interdisciplinary tools and approaches to tackle future problems in the environmental sciences.