Cell, Tissue and Animal Core

细胞-组织-动物核心 摘要 这个核心的主要目标是提供原代细胞培养和动物模型 调查人员将实施关于慢性艾滋病毒感染/老龄化/购物车影响的研究项目 脑部疾病。长期以来，我们一直为Center提供优质的啮齿动物和人类原代细胞 调查员使用。我们新开发了人源化的NSG小鼠模型，并在 神经艾滋病的其他啮齿动物模型。我们有多种技术和分析方法来研究 啮齿动物和非人类灵长类动物模型，询问链和相关研究的宝贵工具 问题。此核心提供和支持的单元和模型在我们的许多 研究人员的神经艾滋病研究。通过使用成像和Omics核心，研究人员可以研究 动物模型中疾病的进展和神经病理学的分子决定因素。国家的现状 还提供了分析的艺术方法。这个核心的总体目标是1)支持中心 研究人员通过提供原代人类和啮齿动物的血液以及动物的脑细胞和组织 神经艾滋病的模型；2)与连锁研究人员合作研究慢性艾滋病的动物模型 艾滋病毒中枢神经系统感染，以及3)提供服务/培训原位脑组织分析，量化 SIV/HIV病毒RNA、DNA载量和许多其他免疫学和病毒学分析需要 研究中枢神经系统疾病。

Cell-Tissue-Animal Core Abstract The primary objective of this core is providing primary cell culture and animal models to CHAIN investigators to execute their research projects on the effect of chronic HIV infection/ aging/ cART on brain disease. We have long provided quality primary cells from rodents and humans for Center investigator use. We have newly developed Humanized NSG mouse model and have experience in other rodent models of neuroAIDS. We have multiple techniques and assays enabling the study of both rodent and nonhuman primate models, valuable tools to ask CHAIN and related research questions. The cells and models provided and supported by this Core are essential in many our investigators’ neuroAIDS studies. By employing Imaging and Omics Cores, investigators can study progression of disease and molecular determinants of neuropathology in animal models. State of the art methods of analyses are also provided. The general goals of this core is 1) to support the center investigators by providing primary human and rodent blood and brain cells and tissues from animal models of neuroAIDS; 2) to work with CHAIN investigators in the study of animal models of chronic HIV CNS infection, and 3) to provide services/training in situ brain tissue analyses, quantification for SIV/HIV viral RNA, DNA loads, and many other immunological and virological assays required to study CNS disorders.