Core C: Cardiovascular Pathology Core

核心结构 心血管病理学核心(核心C)将为中心项目提供几个心血管损伤生物标记物的统一和有效的测量。使用最先进的技术，该核心已经建立了标准操作程序(SOP)，用于对一系列生物化学物质、代谢物、细胞因子以及常见和罕见的血细胞进行量化。虽然它负责满足当前项目的现有需求，但核心服务的范围将不限于这些项目的需求。]T将完善正在进行的探索领域，并努力开发新的分析方法，以扩大其全套技术。CORE丰富而充满活力的资源及其研究人员在心血管病理学和毒理学方面的卓越技术也将使其他烟草研究中心能够与A-TRAC对接。核心将设在UofL健康科学中心的Baxter II大楼内。 核心目前拥有最先进的设备来满足其所有需求。在流式细胞仪中，Core拥有LSR II(Becton Dickinson，加利福尼亚州圣何塞)、Acuri C6 II(Becton Dickinson)和MoFlo Legacy细胞分选仪(达科，Carpinteria，CA)。其他基本设备包括Cobas-Mira Plus化学分析仪、CellDyn血细胞分析仪、Chrono-log聚集仪、BioTek SynergyMX平板阅读器和蔡司LSM 510共焦显微镜(有关所有设备的详细信息，请参阅参考资料)。核心人员还可以获得组织学、免疫组织化学和显微成像的其他设备。

CORE STRUCTURE The Cardiovascular Pathology Core (Core C) will provide unified and validated measurements of several biomarkers of cardiovascular injury for Center Projects. Using state-of-the-art techniques, this Core has already established Standard Operating Procedures (SOPs) for the quantification of a range of biochemicals, metabolites, cytokines, and common and rare blood cells. While it is charged to address the existing needs of the current projects, the scope of the Core services will not be limited to the requirements of these projects. ]t will refine ongoing areas of exploration and strive for developing novel assays to expand its repertoire. The fertile and dynamic resources of the Core and the technical excellence of its investigators in cardiovascular pathology and toxicology will also enable other Tobacco Research Centers to interface with A-TRAC. The Core will be housed in the Baxter II building in the Health Sciences Center of UofL. The Core currently has state of the art equipment for all its needs. Among the flow cytometers, the Core has an LSR II (Becton Dickinson; San Jose, CA) an Acuri C6 II (Becton Dickinson) and a MoFlo Legacy cell sorter (Dako, Carpinteria, CA). Other essential equipment include a Cobas-Mira Plus chemistry analyzer, a CellDyn hematology analyzer, a Chrono-log aggregometer, a BioTek SynergyMX plate reader, and a Zeiss LSM 510 confocal microscope (for details of all equipment, see Resources). Additional equipment for histology, immunohistochemistry and microscopic imaging are also available to Core personnel.