Instantaneous 3D imaging of cells and tissues using polarized light microscopy

 描述（由申请人提供）：我们建议开发偏振光场显微镜，用于分析活细胞、组织和无细胞模型系统内的结构动力学。该仪器结合了全面的偏振分析和显微镜样品的3D成像在一个单一的快照使用新的光场成像方法。该项目包括开发硬件、软件和算法，用于捕获偏振光场图像，并将其层析重建为双折射、双衰减和偏振荧光的三维地图。该仪器的开发是由几个应用项目，包括成纤维细胞的细胞外胶原蛋白基质的重塑，在真核细胞内的高阶septin结构的组装和染色体取向不良的起源及其修复精母细胞的减数分裂纺锤体的空间协调。有关该技术的信息，包括在此资助下开发的软件组件和仪器设计，将在一个名为OpenPolScope.org的门户网站上向用户和开发人员提供。

 DESCRIPTION (provided by applicant): We are proposing to develop the polarized light field microscope for analyzing the architectural dynamics inside living cells, tissues and cell-free model systems. The instrument combines comprehensive polarization analysis and 3D imaging of microscopic specimens in a single snapshot using the new approach of light field imaging. The project includes the development of the hardware, software and algorithms required for capturing polarized light field images and their tomographic reconstruction into 3-dimensional maps of birefringence, diattenuation and polarized fluorescence. The instrument development is guided by several application projects including the remodeling of the extracellular collagen matrix by fibroblast cells, the spatial coordination in the assembly of higher order septin structures inside eukaryotic cells and the origins of chromosome malorientations and their repair in the meiotic spindle of spermatocytes. Information about the technology, including software components and instrument design that are developed under this grant, will be made available for users and developers on an established web portal called OpenPolScope.org.