Impacts of Human Movement on Regional Strain Distribution of Diarrheagenic E.coli

描述（由申请人提供）：本提案描述了埃默里大学罗林斯公共卫生学院环境卫生系助理教授凯伦·利维博士的职业发展和培训计划。Levy博士的职业目标是成为传染病流行病学和生态学的独立调查员，重点是肠道疾病。K 01指导职业发展奖将使她能够建立在持续的合作基础上，与新的导师建立关系，并保护时间来制作科学手稿，这将巩固她已经很强的出版记录。Levy博士将通过以下方式接受分子流行病学和细菌遗传学方面的培训：（1）参加一系列短期课程;（2）获得微生物基因组学方面的经验和培训;以及（3）接受高级教师团队的指导。通过这项培训计划，Levy博士将提高她整合社会，流行病学和分子数据的能力，以了解传染病传播和传播的机制。她的指导团队包括传染病流行病学专家：埃默里大学的Uriel Kitron博士和Les真实的博士以及密歇根大学的Betsy Foxman博士。工作环境：埃默里大学为Levy博士提供了一个很好的场所，让他发展成为一名独立的研究人员，该大学已经表示致力于Levy博士的职业发展。埃默里大学在水和健康研究以及疾病生态学方面的优势使其特别适合肠道疾病流行病学的职业生涯。研究：拟议的研究项目将使用精细尺度的遗传数据，以了解在多个空间尺度上的传播过程，从地方到全国范围内，影响E. coli的分布。大肠杆菌菌株在厄瓜多尔埃斯梅拉达斯省博尔邦地区。本研究的目的是调查城市和农村地区之间的人类旅行在确定致病性大肠杆菌流行株分布中所起的作用。杆菌这是 重要的是，因为循环中的菌株会影响天真人群中的腹泻病的发生。研究方法结合了经典的流行病学病例对照研究设计，全基因组测序，系统发育分析，以及人类运动的数据，以解决以下具体目标：（1）调查旅行作为肠道疾病的危险因素，特别是大肠杆菌。（2）研究区域性人口中心作为大肠杆菌共同中心的作用。在边远农村流行的大肠杆菌菌株;（3）调查 致病性E.大肠杆菌菌株和全国范围内的人员流动模式。本研究将扩大透镜，通过它来查看和设计最佳控制策略，控制肠道疾病。研究结果将提供有关投资基础设施以控制密集人口中心病原体的见解，是否也可以通过限制不同菌株的区域传播来影响偏远农村地区的条件。杆菌

DESCRIPTION (provided by applicant): This proposal describes a career development and training plan for Dr. Karen Levy, Assistant Professor in the Department of Environmental Health at Emory University's Rollins School of Public Health. Dr. Levy's career goal is to become an independent investigator in the epidemiology and ecology of infectious diseases with a focus on enteric diseases. The K01 mentored career development award will allow her to build on ongoing collaborations, develop relationships with new mentors, and have protected time to produce scientific manuscripts that will bolster her already strong publication record. Dr. Levy will receive training in molecular epidemiology and bacterial genetics by (1) participating in a series of short courses; (2) gaining experience and training in microbial genomics; and (3) receiving mentoring from an exceptional team of senior faculty. Through this training program, Dr. Levy will increase her capacity to integrate social, epidemiological, and molecular data towards understanding mechanisms of infectious disease transmission and spread. Her mentoring team includes experts in infectious disease epidemiology: Drs. Uriel Kitron and Les Real at Emory University and Dr. Betsy Foxman at University of Michigan. ENVIRONMENT: Emory University provides an excellent venue for Dr. Levy to develop a career as an independent investigator and the university has signaled its commitment to Dr. Levy's career development. Emory's strengths in water and health studies and disease ecology make it especially suited to incubation of a career in the epidemiology of enteric diseases. RESEARCH: The proposed research project will use fine-scale genetic data to understand transmission processes at multiple spatial scales, from local to country-wide, affecting the distribution of diarrheagenic E. coli strains in the Borbon region of the Ecuadorian province of Esmeraldas. The goal of the research is to investigate the role that human travel between urban and rural regions plays in determining the distribution of circulating strains of pathogenic E. coli. This is important because the strains in circulation affect the occurrence of diarrheal illness in na�ve populations. The research approach combines a classic epidemiological case-control study design with whole genome sequencing, phylogenetic analysis, and data on human movement to address the following Specific Aims: (1) Investigate travel as a risk factor for enteric diseases, specifically diarrheagenic E. coli; (2) Examine the role of a regional population center as a common hub of diarrheagenic E. coli strains circulating in outlying rural villages; (3) Investigate parallels between genetic similarity of pathogenic E. coli strains and human movement patterns on a country-wide scale. This research will widen the lens by which to view and design optimal control strategies for the control of enteric diseases. The results will provide insights into whether investing in infrastructure to control pathogens in dense population centers could also affect conditions in outlying rural areas by limiting regional circulation of diverse strains of diarrheagenic E. coli.