Bioanalytical/Mass Spectrometry/Proteomics Core

生物分析/质谱/蛋白质组学核心（“MS/蛋白质组学核心”）为范德比尔特消化疾病研究中心（VDDRC）的研究人员提供了具有成本效益的最先进仪器和专业知识。该核心是利用位于范德比尔特质谱研究中心（MSRC）的技术人员和仪器设施的专业服务核心之一。该核心将用于小分子代谢物的鉴别和定量以及蛋白质的鉴别和表征。在过去的五年中，46名VDDRC研究人员在消化系统疾病相关项目中广泛使用了该核心，例如协助开发分析方法或实验设计，进行复杂蛋白质样品的分析。核心开发标准操作程序，并维护特定测定、仪器性能和维护历史的质量控制（QC）记录。核心人员为研究人员进行分析，并在MS的理论和实践方面对学生和研究员进行培训。MS核心组件作为一个开放获取的设施运行，用户通常在其中准备他们的样品并在需要时操作仪器。在研究者和核心工作人员协商确定最合适的分析服务类别后，将蛋白质组学样本提交给核心工作人员进行分析。工作人员负责样品处理、分析和提交用于蛋白质组学分析的样品的数据报告的所有方面。行政工作人员监测调查人员使用仪器设施的情况，并编写供行政核心使用的使用情况报告。MSRC核心有18个质谱仪可供用户使用，此外还有用于2D差异凝胶电泳和凝胶成像的专用设施。核心的具体目标是：1）提供高质量的GC/MS，串联LC/MS和MALDI/TOF质谱服务，用于分析小分子代谢物; 2）提供蛋白质组学服务，用于识别和表征单个蛋白质和更复杂的组织特异性蛋白质组;（3）提供质谱分析专业知识，用于检测开发和验证; 4）协助用户进行数据分析;以及5）为学生和研究员提供生物医学质谱和蛋白质组学方面的高级培训。核心的目标是提高研究者预防，诊断或治疗人类消化系统疾病相关疾病的能力。

The Bioanalytical/Mass Spectrometry/Proteomic Core ("MS/Proteomics Core") provides cost-effective, state of-the-art instrumentation and expertise to investigators in the Vanderbilt Digestive Disease Research Center (VDDRC). This core is among the specialized service cores utilizing the technical personnel and instrument facilities located in the Vanderbilt Mass Spectrometry Research Center (MSRC). This core will be used for identification and quantitation of small molecule metabolites and identification and characterization of proteins. Forty six (46) VDDRC investigators have used the core extensively during the previous five years for digestive disease related projects, such as assistance in developing analytical methods or experimental design, performing analysis of complex protein samples. The core develops standard operating procedures, and maintains quality control (QC) records on particular assays, instrument performance, and maintenance history. Core personnel perform assays for investigators and train students and fellows in the theoretical and practical aspects of MS. The MS Core component is run as an open-access facility in which users generally prepare their samples and operate the instruments if they so desire. Proteomics samples are submitted to the core for analysis by proteomics staff after consultations between the investigator and core staff have determined the most appropriate class of analytical service. Personnel handle all aspects of sample processing, analysis, and data reporting of samples submitted for proteomics analysis. Administrative staff monitors the use of the instrument facilities by investigators and prepare reports on utilization for use by the Administrative Core. The MSRC cores have 18 mass spectrometers available to users, in addition to specialized facilities for 2D-differential gel electrophoresis and gel imaging. The Specific Aims of the Core are to: 1) provide high-quality GC/MS, tandem LC/MS, and MALDI/TOF mass spectrometry services for analysis of small molecule metabolites; 2) provide proteomics services for identification and characterization of individual proteins and more complex tissue-specific proteomes; (3) provide analytical expertise in mass spectrometry for assay development and validation; 4) assist users with data analysis; and 5) provide advanced training in biomedical mass spectrometry and proteomics to students and fellows. The goal of the Core is to enhance investigator abilities to prevent, diagnose or treat human digestive disease-related disorders.