Generating new mouse mutant strains

在过去的一年里，我们的研究核心与美国国立卫生研究院的10个研究单位合作，创造了新的胚胎干细胞系(24个项目，其中大多数使用CRISPR/Cas9转基因)和新的小鼠模型(36个项目，包括6个CRISPR/Cas9合子注射)。这项研究包括建立模型，研究与RNaseH功能障碍相关的疾病，免疫系统中的信号通路相互作用，T细胞发育，造血，干细胞生物学，早期胚胎的表观遗传，调节基因组稳定性的机制，RNA代谢，骨和细胞外基质中的遗传疾病，基因调节和增强功能，神经系统发育，铁代谢和疟疾风险，以及内分泌和癌症遗传学。我们还对4个小鼠品系进行了冷冻保存，从转基因小鼠中获得了14个新的胚胎干细胞系，并重新获得了4个品系的小鼠。 在过去的一年里，我们进一步发展了进行受精卵注射(核和细胞质)的专业技术，并向NICHD研究界提供了这些服务。

During the last year our research core has worked with ten NIH research units to generate novel embryonic stem cell lines (24 projects, most of them using CRISPR/Cas9 transfections) and new mouse models (36 projects, including 6 CRISPR/Cas9 Zygote injections). This research included establishment of models to study disease associated with RNAse H dysfunction, signal pathway interactions in the immune system, T cell development, hematopoiesis, stem cell biology, epigenetic inheritance in the early embryo, mechanisms regulating genomic stability, RNA metabolism, genetic disorders in bone and extracellular matrix, gene regulation and enhancer function, nervous system development, iron methabolism and malaria risk, and endocrine and cancer genetics. We also performed cryopreservation of 4 mouse lines, derived 14 novel embryonic stem cell lines from genetically altered mice, and re-derived 4 mouse strains. In the past year we developed further expertise to perform zygote injections (nuclear and cytoplasmic) and offered these services to the NICHD research community.