ZFIN: The Zebrafish Model Organism Database

发现人类基因组中数以万计的基因的功能是 了解人类生物学和疾病。遗传模式生物，包括斑马鱼，在 这一发现过程，是因为基因分析可以将基因序列和功能联系起来。模式生物 像ZFIN这样的数据库提供了建立这种连接所需的工具。 斑马鱼已经成为研究脊椎动物生物学的首要生物。强大的技术允许 快速高效地产生和恢复影响协调发育模式的基因的突变， 器官发生、生理和行为。通过产生转基因斑马鱼来研究基因功能很容易， 通过用吗啉反义寡核苷酸敲除基因功能，或通过改变基因功能 基因组编辑。基因组已经测序，大约50%的蛋白质编码基因已经被测序 被靶向基因敲除技术突变。正在进行或计划中的大型项目将 产生关于基因组中几乎所有基因和基于序列的功能元件的功能数据。 多个突变和基因敲除可以在同一个体中组合，以研究基因修饰物和 其他遗传交互作用。这些基因中的大多数功能在脊椎动物群体中是保守的。 因此，对斑马鱼突变的分析提供了对其他脊椎动物基因功能的洞察，包括 人类。 ZFIN的长期目标是a)成为社区数据库资源，供实验室使用 斑马鱼，b)开发和支持整合了遗传、基因组、发育和生理的斑马鱼 信息，c)维护斑马鱼研究信息的最终参考数据集，d)将 信息广泛到其他模式生物和人体数据库中的相应数据，e)以便于 使用斑马鱼作为人类生物学的模型，以及f)帮助服务于生物医学的广泛需求 研究社区。 该项目将继续并扩大斑马鱼研究数据的管理，为 斑马鱼人类疾病模型，扩展和整合到其他数据库的链接，以及维护和更新 斑马鱼的参考基因组。这项工作将为研究人员提供强有力的基因关联手段 序列和功能，从而促进人类基因功能和疾病以及跨物种的研究 基因组组织和进化的分析。

Discovering the functions of the tens of thousands of genes in the human genome is a required step for understanding human biology and disease. Genetic model organisms, including zebrafish, play a critical role in this discovery process, because genetic analysis can connect gene sequence and function. Model organism databases, like ZFIN, provide tools required to make this connection. The zebrafish has emerged as a premier organism to study vertebrate biology. Powerful techniques allow rapid efficient generation and recovery of mutations affecting genes that orchestrate developmental patterning, organogenesis, physiology, and behavior. It is easy to study gene function by generating transgenic zebrafish, by knocking down gene function with morpholino antisense oligonucleotides, or by altering gene function by genome editing. The genome has been sequenced and about 50% of the protein coding genes have been mutated by targeted gene knockout technology. Large-scale projects are underway or planned that will produce functional data about almost all the genes and sequence-based functional elements in the genome. Multiple mutations and gene knockdowns can be combined in the same individual to study gene modifiers and other genetic interactions. The functions of most of these genes are conserved among vertebrate groups. Thus, analysis of zebrafish mutations provides insights into gene functions in other vertebrates, including humans. The long term goals for ZFIN are a) to be the community database resource for the laboratory use of zebrafish, b) to develop and support integrated zebrafish genetic, genomic, developmental, and physiological information, c) to maintain the definitive reference data sets of zebrafish research information, d) to link this information extensively to corresponding data in other model organism and human databases, e) to facilitate the use of zebrafish as a model for human biology, and f) to help serve the broad needs of the biomedical research community. This project will continue and expand curation of zebrafish research data, develop expanded support for zebrafish models of human disease, expand and integrate links to other databases, and maintain and update the zebrafish reference genome. This work will provide a powerful means for researchers to associate gene sequence and function, thus facilitating studies of human gene function and disease as well as cross-species analyses of genome organization and evolution.