Einstein Mount Sinai Diabetes Research Center

稳定同位素和代谢组学核心(SIMC)的目标是为爱因斯坦提供 西奈糖尿病研究中心研究人员性价比高、效率高、健壮 用于测定代谢物/脂类的平台，用于覆盖联锁代谢物 和脂质途径，并有助于提高我们对改变燃料的条件的理解 胞质和线粒体代谢途径的利用和生物合成，即糖酵解， 戊糖和三羧酸(TCA)循环。为了在一个设施中实现这些目标，SIMC 已更新其技术基础设施，包括最新的SeaHorse XFe24和XFe96 用于实时评估糖酵解和线粒体能量学的海马通量分析仪， 最新的Sciex 6500+QTRAP具有选择性离子迁移率，用于靶向LC/MS代谢产物和 脂肪组学分析，并将取代其Orbitrap/LTQ高质量精度仪器 最新岛津LC-9030四极飞行时间与Nexera UPLC无目标 代谢组学和脂肪组学分析。这些最新的添加增加了高质量分辨率 Waters GC-TOF/MS和两台道尔顿分辨率安捷伦单四极GC/MS联用机 实现以下目标： 1.确定用于诊断/表征糖尿病的代谢组/脂体学特征 细胞、组织和生物体液(血浆、尿液、 凳子等)。 2.进行体内稳定同位素底物分析以确定蛋白质合成速率， 脂肪生成、外周葡萄糖处置、肝脏葡萄糖循环、葡萄糖-甘油循环 和Cori循环，高能磷酸(ATP，肌酸磷酸)的周转，并在体外 代谢途径的稳定同位素通量剖析。 3.进行糖酵解的评估(细胞外酸化率、糖酵解ATP 生产速率)和线粒体耗氧量(线粒体呼吸和 组织外植体、原代分离和组织培养中的线粒体ATP产生率 使用海马生物科学通量分析仪的细胞。 4.向刚果民主共和国调查人员及其学生、研究员和技术人员提供咨询和指导 在设计和解释通量组学、代谢组学/类脂组学和计算 分析的目的是阐明葡萄糖和脂肪的分子基础 人体和动物模型的动态平衡。

The goal of the Stable Isotope and Metabolomics Core (SIMC) is to provide to Einstein-Mount Sinai Diabetes Research Center research investigators cost effective, efficient and robust platforms for the determination of metabolites/lipids designed to cover interlocking metabolite and lipid pathways, and help to improve our understanding of conditions which change fuel utilization and biosynthesis in cytosolic and mitochondrial metabolic pathways, i.e., glycolysis, pentose and tricarboxylic acid (TCA) cycles. To accomplish these goals in one facility, the SIMC has updated its technology infrastructure to include the latest Seahorse XFe24 and XFe96 Seahorse Flux Analyzers for assessment of glycolytic and mitochondrial energetics in real time, the latest Sciex 6500+ QTRAP with Selexion ion mobility for targeted LC/MS.MS metabolite and lipidomic analyses, and is due to replace its Orbitrap/LTQ high mass accuracy instrument with the latest Shimadzu LC-9030 quadrupole Time of Flight with Nexera UPLC for untargeted metabolomic and lipidomic analyses. These latest additions add to the high mass resolution Waters GC-TOF/MS and two unit Dalton resolution Agilent single quadrupole GC/MS machines to accomplish the following aims: 1. To determine metabolomic/lipidomic profiles for the diagnosis/characterization of physiological and pathophysiological states in cells, tissues and biofluids (plasma, urine, stool, etc.). 2. To perform in vivo stable isotope substrate assays to determine rates of protein synthesis, lipogenesis, peripheral glucose disposal, hepatic glucose recycling, glucose-glycerol cycling and Cori cycling, high energy phosphate (ATP, creatine phosphate) turnover, and in vitro stable isotope flux dissections of metabolic pathways. 3. To perform assessments of glycolysis (extracellular acidification rates, glycolytic ATP production rates) and mitochondrial oxygen consumption (mitochondrial respiration and mitochondrial ATP production rates) in tissue explants, primary isolated and tissue culture cells using Seahorse Biosciences Flux Analyzers. 4. To advise and instruct ES-DRC investigators and their students, fellows and technical staff in the design and interpretation of fluxomics, metabolomics/lipidomics and computational analyses with the aim of elucidating the molecular basis underlying glucose and lipid homeostasis in humans and animal models.