Cell Culture and Cell Phenotyping

资源/服务核心：细胞培养和细胞表型模块 摘要 细胞培养和细胞表型模块的目标是简化细胞培养和表型 视觉研究人员的分析。为实现这一目标，本模块将支持维护和 严格研究以NEI为重点的广泛研究所需的细胞系的鉴定 活动，包括所有由NEI资助的研究项目以及新的协作和试点项目。导演和 本单元的工作人员将提供培训、技术支持和关键设备的维护，以促进 科学严谨、前沿、视野研究。 本单元的细胞培养部门将提供以下基本服务： 1)永生化细胞系的常规维护。 2)细胞系超低温保存库存的维护。 3)原代角膜和视网膜细胞系的建立。 4)支原体和内毒素污染常规筛查。 5)短串联重复序列DNA图谱用于细胞系鉴定。 该单元的细胞表型鉴定部门将提供以下全面的分析服务： 1)流式细胞术。 2)细胞分选。 3)高通量细胞分析。 4)实时细胞代谢图谱。 5)微泡定量和大小测定。 总而言之，这些服务将为所有NEI资助的校园视觉研究人员提供最先进的 将提高他们的实验效率并支持生成高质量、量化的 为视觉研究的各个方面提供数据。

Resource/Service Core: Cell Culture and Cell Phenotyping Module Abstract The goal of the Cell Culture and Cell Phenotyping Module is to streamline cell culture and phenotyping analyses for vision researchers. To accomplish this goal, this Module will support the maintenance and authentication of cell lines that are required for rigorous study of a wide range of NEI-focused research activities, including all NEI-funded research projects and new collaborative and pilot projects. The director and staff of this Module will provide training, technical support, and maintenance of key equipment to facilitate scientifically rigorous, cutting edge, vision research. The Cell Culture arm of this module will provide the following essential services: 1) Routine maintenance of immortalized cell lines. 2) Maintenance of cryopreserved stocks of cell lines. 3) Establishment of primary corneal and retinal cell lines. 4) Routine screening for mycoplasma and endotoxin contamination. 5) Short tandem repeat DNA profiling for cell line authentication. The Cell Phenotyping arm of this module will provide the following comprehensive analytical services: 1) Flow cytometry. 2) Cell sorting. 3) High throughput cellular analysis. 4) Live cell metabolic profiling. 5) Microvesicle quantification and size determination. Collectively, these services will provide all NEI-funded vision researchers on campus with state of the art resources that will increase their experimental efficiency and support the generation of high quality, quantitative data for all aspects of vision research.