Metabolomics Core

核心3项目摘要 新陈代谢核心 代谢核心(核心#3)支持由PROGRAM设计的代谢组学和蛋白质组学实验 将项目赠款(PPG)调查员作为其拟议研究的组成部分。这些大规模的分子 PPG中的所有四个项目都使用了数据分析策略，提供了协同联结点， 由Core#3推动，跨项目交叉识别起重要作用的代谢物和蛋白质 在肺癌生物学和免疫学中的作用。核心#3成员在癌症方面拥有丰富的经验 代谢，代谢组学，蛋白质组学，液-质联用，气相色谱-质谱法 光谱、与调查人员的合作、与数据科学家(包括Core的成员)的合作 #4)，以及客户服务。这些努力将有助于项目从开始到完成(即从 假设到出版)。CORE#3将支持质谱学实验设计的优化- 以组学为基础(目标1)，包括实施先前公布的战略和制定 新的样品制备方法，包括气相色谱和液相色谱的分离，以及质量 光谱分析以支持PPG调查人员所需的实验。将通过以下方式获取数据集 非靶向代谢组学、发现蛋白质组学、翻译后修饰分析和靶向 利用最新分离和质谱仪分析代谢物和蛋白质的研究进展 莫菲特癌症中心的设备(目标2)。3号核心成员将为质量保证/质量做出贡献 控制为每个项目获取的数据，并与PPG调查员和其他PPG核心合作以 生成高质量的数据集并开发数据分析策略，以最大限度地提高组学的可用性和使用率 跨PPG项目的数据(目标3)。总而言之，核心3号将对拟议的 肺肿瘤生物学和肿瘤免疫细胞分子机制的研究进展 相互作用，最终可能揭示潜在的生物标志物和新的治疗靶点，有待进一步 在PPG项目提出的功能研究中进行了调查。

CORE 3 PROJECT SUMMARY METABOLISM CORE The Metabolism Core (Core #3) supports the metabolomics and proteomics experiments designed by program project grant (PPG) investigators as integral parts of their proposed research. These large-scale molecular data analysis strategies are used by all four projects in this PPG, which provides synergistic linkage points, facilitated by Core #3, across projects for cross identification of metabolites and proteins that play important roles in lung cancer biology and immunology. Core #3 members have extensive experience in cancer metabolism, metabolomics, proteomics, liquid chromatography-mass spectrometry, gas chromatography-mass spectrometry, collaboration with investigators, collaboration with data scientists (including members of Core #4), and customer service. These efforts will contribute to projects from inception to completion (i.e. from hypothesis to publication). Core #3 will support optimization of experimental design for mass spectrometry- based omics (Aim 1), which includes implementation of previously published strategies and development of novel methods for sample preparation, separations including gas and liquid chromatography, and mass spectrometry to support the experiments required by PPG investigators. Datasets will be acquired with untargeted metabolomics, discovery proteomics, analysis of post-translational modifications, and targeted assay development for metabolites and proteins utilizing state-of-the-art separations and mass spectrometry equipment at Moffitt Cancer Center (Aim 2). Core #3 members will contribute to quality assurance/quality control for data acquired for each project and collaborate with PPG investigators and other PPG Cores to produce high quality datasets and develop data analysis strategies to maximize availability and usage of omics data across PPG Projects (Aim 3). In summary, Core #3 will make a significant contribution to the proposed research by elucidating molecular mechanisms underlying lung tumor biology and tumor-immune cell interactions, which may ultimately reveal potential biomarkers and novel treatment targets to be further investigated in functional studies proposed by the PPG Projects.