课题基金 / 基金详情

Gene Transfer Core, NIDCR-DIR

Gene Transfer Core, NIDCR-DIR
基因转移核心，NIDCR-DIR
批准号：
10685891
负责人：
Ashok Kulkarni
金额：
$77.91万
依托单位：
NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/10685891
关键词：
Biological AssayBiopsyClone CellsClustered Regularly Interspaced Short Palindromic RepeatsConsultationsCryopreservationEmbryoEmbryo TransferGene TargetingGene TransferGenerationsGenesGenomic DNAGenotypeInjectionsKnock-in MouseKnock-outKnockout MiceMusNational Institute of Dental and Craniofacial ResearchPrincipal InvestigatorResearchResearch PersonnelServicesTailTransgenic MiceTransgenic Organismsbaseembryonic stem cellexperimental studygain of functionloss of functionmouse modelpreservationsperm cellsperm cryopreservationtool

项目摘要

项目成果

Ashok Kulkarni的其他基金

相似基金

相关文献

中文摘要
翻译
在2022财年，基因转移中心成功提供了以下服务： 原核注射服务：注射13个基于CRISPR的基因编辑构建体，产生19个转基因株系。 基于CRISPR的基因编辑服务：对于基于CRISPR的构建体，产生KO和KI小鼠的平均效率分别为20%和23%。 冷冻保存和IVF服务：为8名主要研究者（10名研究者）冷冻保存了34只小鼠模型的精子。来自所有小鼠模型的精子储存在两个单独的建筑物中。解冻16根精子管，进行20次IVF。 基因分型服务：纯化480份尾部活检标本，并通过PCR分析进行基因分型。
英文摘要
In FY22, the Gene Transfer Core successfully provided following services: Pronuclear injection service: 13 CRISPR-based gene editing constructs were injected to generate 19 transgenic lines. CRISPR-based gene editing service: The average efficiency for generating KO and KI mice for CRISPR-based constructs was 20% and 23%, respectively. Cryopreservation and IVF services: Sperm from 34 mouse models were cryopreserved for 8 Principal investigators (10 investigators). Sperm from all mouse models were stored in two separate buildings. 16 sperm straws were thawed for 20 IVF sessions. Genotyping service: 480 tail biopsies were purified and used for genotyping by PCR analysis.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Molecular Roles of TGF-beta Signaling in Salivary Glands and Oral Cancer
Molecular Roles of Cdk5 in Neuronal Functions and Pain Signaling
Molecular Genetics of Craniofacial Development and Disease
Characterization of Molecular Pathways in Chronic Pain Conditions
海外基金