FACTORS IMPORTANT FOR PATHOGENICITY IN CAMPYLOBACTER JEJUNI: BIOTERRORISM

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 空肠弯曲杆菌是一种重要的新兴细菌性食源性人类病原体，其引起一系列疾病，包括胃肠炎、直肠炎、败血症、流产、脑膜炎和上行性麻痹。 家禽、猪肉、牛肉或其他食物和水中的800种微生物就能致病。 每1000名接触者中就有1人患上衰弱性神经系统疾病。 目前对C.空肠感染 家畜是人类细菌的重要来源，但从动物转移到人类的机制尚未完全了解。鸡、牛和猪携带C。空肠作为胃肠道肠道，只有很少的发展临床疾病和病理由于细菌。我们研究的长期目标是从食物链中消除弯曲杆菌。 该项目的长期目标是开发蛋白质组学方法，可以作为MSU研究界，兽医界，食用动物生产商，美国农业部，医学界和美国生物恐怖主义研究的资源。 我们将与中心的合作者合作，建立使用自动化高通量二维电泳方法对弯曲杆菌分离株进行分型的方法。 我们已经收集了2500多个来自患有弯曲杆菌疾病的动物和人类的分离株，以及来自正常动物和环境的分离株。将为这些菌株中的一些菌株建立蛋白质表达谱。一旦生成，将比较模式，并探索独特的蛋白质的身份和功能，并生成数据库。 大量的应用程序将产生的信息，但我们将集中在确定致病菌株共享的毒力属性，而不是在非致病性病原体。 从长远来看，我们将开发致病性C的特征。空肠分离株，可以作为快速筛选的特点，在爆发和其他应用分离。 这将作为利用蛋白质组学开发病原微生物自动快速筛选的示范项目。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Campylobacter jejuni is a significant emerging bacterial food borne pathogen of humans that causes a spectrum of diseases including gastroenteritis, proctitis, septicemia, abortion, meningitis, and ascending paralysis. As few as 800 organisms in poultry, pork, beef or other foods and water can cause disease. 1 out of 1000 exposed people develop debilitating neurological disease. Little is known about the molecular mechanisms involved in establishment of a C. jejuni infection. Domestic animals are a significant source of the bacterium for humans, but the mechanisms of transfer from animals to humans are not fully understood. Chickens, cattle and pigs carry C. jejuni as a GI commensal and only rarely develop clinical disease and pathology due to the bacterium. The long-term goal of our research is to eliminate Campylobacter from the food chain. The long-term goal of this project is to develop proteomic methods that could serve as a resource for the MSU research community, the veterinary community, food animal producers, USDA, the medical community, and US bioterrorism research. We will work with our collaborators in the Center to establish methods for typing Campylobacter isolates using automated high throughput 2 dimensional electrophoresis methods. We have assembled over 2500 isolates from animals and humans with disease due to Campylobacter, as well as isolates from normal animals and the environment. Protein expression profiles will be established for some of these strains. Once generated, patterns will be compared and unique proteins explored for identity and function, and a database will be generated. Numerous applications will arise from the information generated, but we will focus on identifying virulence attributes shared by isolates causing disease and not present in nonpathogenic commensals. In the long term we will develop a profile for pathogenic C. jejuni isolates that could serve as a rapid screen for characterizing isolates in outbreaks and other applications. This will serve as a demonstration project for developing automated rapid screens for pathogenic microorganisms using proteomics.