Core 3: Biocontainment Research Support Services Core

摘要--生物遏制研究支持服务核心(核心3) 杜克大学RBL的中心任务是支持开发所需的基础和翻译研究 针对新出现/再次出现的感染和生物防御的药物、诊断和疫苗。在此过程中提供支持 奖励将使我们能够扩展我们现有的服务，以完善我们的小动物模型计划，从而提供 为研究界提供更广泛的服务。它还将使我们的病毒学计划 有效，特别是通过开发我们在杜克大学临床实验室的直接工作流程 除了依赖第三方来源，特别是对于快速出现的病毒变种。第三，我们将 扩大我们在BSL-3套件中执行的免疫分析的范围，如活细胞流分选和 细胞因子和生物标志物图谱的快速评估。这项生物遏制研究支持的目标 服务核心(核心3)：1)使用BSL-3建立、维护和提供小动物挑战模型 通过Duke RBL动物模型研究支持服务；2)维护和提供高质量- 通过Duke RBL控制需要BSL-3条件的选定试剂和病原体的病毒库存 病毒学研究支持服务；以及3)在BSL-3通过体液提供全面的免疫监测， 通过杜克大学RBL免疫学和流式细胞术研究支持的细胞和生物标记物分析 服务。这三个目标的实现将使我们能够扩展我们所能提供的能力和服务 不仅提供给杜克大学社区，也提供给我们邻近的机构和国家RBL网络。

ABSTRACT- Biocontainment Research Support Services Core (Core 3) The central mission of the Duke RBL is to support the basic and translational research necessary to develop drugs, diagnostics, and vaccines for emerging/reemerging infections and biodefense. Support through this award will allow us to expand our existing services to refine our small animal models programs, thus providing a wider range of services offered to the research community. It will also render our virology program more effective, particularly through the development of a direct workflow from our clinical laboratories at Duke, in addition to relying on third party sources, particularly for rapidly emerging viral variants. Thirdly, we will broaden our range of immunological assays performed in our BSL-3 suites, such as live cell flow sorting and rapid evaluation of cytokine and biomarker profiles. The goals of this Biocontainment Research Support Services Core (Core 3) are to 1) establish, maintain, and provide small animal challenge models with BSL-3 pathogens through the Duke RBL Animal Models Research Support Services; 2) maintain and provide quality- controlled virus inventory of Select Agents and pathogens requiring BSL-3 conditions through the Duke RBL Virology Research Support Services; and 3) provide comprehensive immune monitoring at BSL-3 via humoral, cellular, and biomarker analysis through the Duke RBL Immunology and Flow Cytometry Research Support Services. Implementation of these three Aims will allow us to expand the capabilities and services that we can offer not only to the Duke community, but also to our neighboring institutions and the national RBL network.