Elucidating the mechanism behind oscillation between glycolysis and gluconeogenesis

项目摘要 新陈代谢是一个动态的生化反应网络，能够适应不断变化的环境。在 改变葡萄糖的利用率、糖酵解和糖异生支持全身葡萄糖稳态。在 本论文研究了哺乳动物糖酵解和异生的调控和协调， 在动态营养环境下的细胞。该补充申请的目标是获得资金， 在2023年夏天支持一位有才华和有动力的本科生研究员10周。她 将测量哺乳动物细胞在动态营养条件下的糖酵解和糖异生速率。 通过采用生物化学测定和质谱法对环境进行分析。她将由PI指导， 一名博士生将训练她进行细胞培养实验，代谢测量和分析 结果她将加入加州大学洛杉矶分校校园的Bruins-In-Genomics（BIG）暑期项目， 视野和发展专业技能。她将与PI的研究小组分享她的研究成果， 校园里的其他研究人员通过在2023年BIG研究研讨会上展示她的项目。这些活动 将进一步促进本科生研究人员对生物医学科学的热情，并作为一块垫脚石 让她成为未来的STEM领袖

Project Summary Metabolism is a dynamic network of biochemical reactions capable of adapting to changing environments. In changing glucose availability, glycolysis and gluconeogenesis support systemic glucose homeostasis. In the parent award, we investigate the regulation and coordination of glycolysis and gluconeogenesis in mammalian cells under dynamic nutrient environments. The goal of this supplement application is to acquire funding to support one talented and motivated undergraduate student researcher for 10 weeks in the summer of 2023. She will measure the rates of glycolysis and gluconeogenesis in mammalian cells under dynamic nutrient environments by employing biochemical assays and mass spectrometry. She will be mentored by the PI and one PhD student who will train her to perform cell culture experiments, metabolic measurements, and analyze results. She will join the Bruins-In-Genomics (BIG) Summer Program on UCLA campus to broaden her scientific horizons and develop professional skills. She will share her research outcomes with the PI’s research group and other researchers on campus by presenting her project at the 2023 BIG Research Symposium. These activities will further the undergraduate student researcher’s passion for biomedical science and act as a steppingstone for her to become a future STEM leader.