DEVELOPMENT OF RAMAN CLINICAL INSTRUMENT FOR TRANSCUTANEOUS GLUCOSE DETECTION

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 建议的子项目的目标是在我们之前在组织模体和动物模型中成功应用的基础上，将拉曼光谱建立为一种可行的临床工具，用于体内血糖测量。然而，生物组织发出的光谱信号往往很弱，因此有效的采集是必不可少的。只有大约1010个入射光是拉曼散射的，这严重限制了数据采集速率。可以传递到给定组织区域的激发光功率受到诸如过热等不良影响的限制。因此，最大限度地增加所收集的光线是很重要的。虽然我们能够使用离轴镀金抛物面反射镜显著提高台式系统的光收集效率，但临床仪器的占地面积要求需要使用基于光纤的激发和收集设备。为了实现这一目标，我们设计了一个紧凑的光谱系统，它包括一个可调谐激光器和一个宽带光源(这两个光源分别对荧光去除和浊度校正至关重要)，以及一个带有复合抛物面聚光器(CPC)的柔性光纤探头。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of the proposed subproject is to establish Raman spectroscopy as a viable clinical tool for in vivo measurement of blood glucose, based on our previous successful application in tissue phantoms and animal models. However, the spectral signals emanating from biological tissue are often weak, and therefore efficient collection is essential. Only approximately 1010 of the incident light is Raman scattered, severely limiting data-acquisition rates. The excitation light power that can be delivered to a given area of tissue is limited by undesirable effects such as overheating. It is therefore important to maximize the light collected. While we were able to significantly enhance light collection efficiency in a bench-top system using an off-axis gold coated paraboloidal mirror, the footprint requirements of the clinical instrument necessitate the use of a fiber optic-based excitation and collection device. To accomplish this, we have designed a compact spectroscopic system, that incorporates a tunable laser and a broadband source (which are critical for fluorescence removal and turbidity correction, respectively), alongside a flexible fiber probe capped with a compound parabolic concentrator (CPC).