Biospecimen-Core

摘要 生物标本核心：肺和心脏功能以及衰老是人类健康和寿命的主要决定因素， 分别肺和心脏疾病是世界范围内发病率和死亡率的主要原因 (WHO《2020年全球健康估计》。生物样本核心（BC）将产生高质量的，临床 来自正常人肺和心脏的注释和病理学评价的标本（以及相应的 血管和淋巴结），为创建高分辨率、多模态和 多维衰老图不列颠哥伦比亚省的调查人员将利用他们的专业知识收集，处理， 对肺和心脏衰老的组织样本和原代细胞系进行注释、分类和分配 映射.该核心将擅长于提供已经编目的组织，其缺血诱导的伪影尽可能少， 这是可能的，并符合所有法律的和道德标准，包括广泛的捐赠者同意。BC遵循NIH/NCI最佳 生物储存库的实践，标准操作程序（SOP）到位，以确保最高的 生物样本和临床信息质量符合所有法律的和伦理标准。生物储存库的工作是 通过OSU、Pitt和URMC IRB协议批准，该协议涵盖采购、处理和分销 人类生物标本BC将在整个生命周期内收集人类的整个组织，并将提供完整的 组织、精密切割组织切片（PCTS）和纯化细胞输送至TriState SenNet TMC。与数据一起 分析核心（DAC），BC将使用已建立的元数据收集协议以 一致和可互操作的格式。

ABSTRACT Biospecimen Core: Lung and heart function and aging are major determinants of human health and lifespan, respectively. Combined, lung and heart diseases are the leading cause of morbidity and mortality world-wide (WHO’s Global Health Estimates 2020). The Biospecimen Core (BC) will generate high-quality, clinically annotated, and pathologically evaluated specimens from normal human lung and heart (and corresponding vessels and lymph nodes) to provide the fundamental basis for the creation of high-resolution, multi-modal, and multi-dimensional senescence maps. The BC investigators will use their expertise in collecting, processing, annotating, classifying, and distributing tissue samples and primary cells lines for lung and heart senescence mapping. The core will excel in providing already catalogued tissues with as little ischemia-induced artifacts as possible, and meet all legal and ethical standards including broad donor consent. The BC follows NIH/NCI Best Practices for Biorepositories, with standard operating procedures (SOPs) in place to ensure the highest biospecimen and clinical information quality to meet all legal and ethical standards. The biorepository efforts are approved through OSU, Pitt, and URMC IRB protocols that cover the procurement, processing, and distribution of human biospecimens. The BC will collect whole tissue from humans across the lifespan and will provide whole tissue, precision cut tissue slices (PCTS), and purified cells to the TriState SenNet TMC. Together with the Data Analysis Core (DAC), the BC will use established metadata collection protocols to collect metadata in a consistent and interoperable format.