Animal and Biostatistics Core

摘要 动物和生物统计核心将直接与项目领导人合作，设计、规划、监测和解释 所有的动物实验以及，都提供了特定的小鼠模型和标准化的实验方案 支持整个计划的共同目标：1)了解年龄和遗传背景如何调节 模拟禁食饮食(FMD)和蛋白质限制循环(PRC)对长寿和健康的影响，2) 研究FMD、PRC依赖的细胞保护、再生和复壮机制 造血和神经系统，3)了解FMD与PRC、GH-IGF-1、mTOR和 以测试这种线粒体肽是PFC/PRC介体的假设，具有潜在的 作为禁食/蛋白质限制的模拟物，4)研究依赖半胱氨酸的伽马裂解酶的调节 由GH/IGF-1-mTOR负调节的气体硫化氢的产生，并检验硫化氢是一种 口服液抗肝缺血再灌注损伤保护作用的介导物 化疗对造血干细胞的毒性作用。核心提供了一个集成的框架， 评估各个项目需求的共同结果。 目标1.在项目负责人的指导下，设计、计划和监督动物实验程序。研究是 旨在获得足够的功率/灵敏度，以满足所述目标。所有实验程序 在小鼠身上执行的操作由Core B标准化并执行，以确保在所有 项目。这些因素包括：A)长寿，B)健康(新陈代谢/认知和细胞/分子)，C)压力 抗性和机制研究，D)组织收集/分布。 目标2.为项目协调动物使用。所有通过核心B获得或产生的动物将被安置在 位于南加州大学雷·R·伊拉尼音乐厅的最先进的隔膜设施。单独的专用动物休息室 对于繁殖、长寿或抗逆性的专有用途，将确保生物的一致性 所有研究的环境、噪音控制和安全。

Abstract The Animal and Biostatistics Core will work directly with Project Leaders to design, plan, monitor and interpret all animal experiments, as well as, provide specific mouse models and standardized experimental protocols in support of the overall program's common goals: 1) understand how age and genetic background modulate the effects of fasting-mimicking diets (FMD) and protein restriction cycles (PRC) on longevity and healthspan, 2) study the mechanisms of FMD-, PRC-dependent cellular protection, regeneration and rejuvenation in the hematopoietic and nervous systems, 3) understand the link between FMD and PRC, GH-IGF-1, mTOR and humanin to test the hypothesis that this mitochondrial peptide is a PFC/PRC mediator with the potential to serve as a fasting/protein restriction mimetic, 4) study the regulation of the cysteine gamma lyase-dependent production of the gas H2S, negatively regulated by GH/IGF-1-mTOR, and test the hypothesis that H2S is a mediator of the protective effects of FMD on resistance to hepatic ischemia reperfusion injury and chemotherapy-induced toxicity to hematopoietic stem cells. The core provides an integrative framework for assessment of common outcomes across the individual projects needs. Aim 1. Design, plan and monitor animal experimental procedures as directed by Project Leaders. Studies are designed to achieve sufficient power/sensitivity to meet the stated objectives. All experimental procedures performed on mice are standardized and performed by Core B to ensure consistent assessment across all projects. These include: A) Longevity, B) Healthspan (Metabolic/Cognitive and Cellular/Molecular), C) Stress resistance and Mechanistic studies, D) Tissue collection/distribution. Aim 2. Coordinate animal use for program. All animals obtained or generated through Core B will be housed in the state-of-the-art vivarium facility in the Ray R Irani Hall at USC. Individual dedicated animal holding rooms for the exclusive use of either breeding, longevity or stress resistance will ensure consistency of biological environment, noise control and security across all studies.