HMS/BCH Center for Neuroscience Research

摘要 现代神经科学在很大程度上依赖于工具来可视化神经的结构，有时甚至是功能 系统。这包括在几纳米(电子显微镜)的水平上检查结构的工具 那些检查整个人类大脑的(核磁共振)。介于这两个极端之间的是检查细胞的方法 亚室和细胞器、细胞形态、小规模环路组织和整个啮齿动物或 果蝇的大脑。通过机构中心核心拨款支持神经科学研究计划， 我们在哈佛医学院和波士顿儿童医院建立了一个中心成像核心，支持 我们社区的研究旨在了解人类神经系统的结构和功能 在健康动物和人类疾病的模型中。堆芯已经运行了大约6年， 并在我们整个社区推动或直接使研究项目成为可能。核心运行 既有日常使用的交钥匙系统，也有用于慢性、高含量和高吞吐量的专用显微镜 成像，以及用于在组织和细胞中以最高分辨率成像的定制管道。核心有 取得了成功，在27个实验室支持了超过35个NINDS项目。在即将到来的赠款期间， 我们扩大了现有的显微镜设备，并重组和简化了行政管理 和技术支撑结构，提高效率。除了其研究任务外，该中心还将 继续作为神经科学社区和 学习成像技术和方法的教育中心。通过可持续发展 作为该中心的一员，我们希望继续为NINDS研究人员提供高性价比和高效率的服务 同时进一步加深这两个充满活力的神经科学界之间的联系。

Abstract Modern neuroscience relies heavily on tools to visualize the structure, and at times the function, of the nervous system. This includes tools that examine structure at the level of few nanometers (electron microscopy) to those that examine whole human brain (MRI). Between these extremes are approaches to examine cellular sub-compartments and organelles, cellular morphology, small-scale circuit organization, and whole rodent or drosophila brains. Through the Institutional Center Core Grant to Support Neuroscience Research program, we established at Harvard Medical School and Boston Children's Hospital a Center Imaging Core that supports research in our community directed at understanding the structure and function of the nervous system in healthy animals and in models of human disease. The core has been operational for approximately 6 years, and has facilitated or directly made possible research projects throughout our community. The core operates both turnkey systems for daily use, specialized microscopes for chronic, high-content, and high-throughput imaging, as well as custom pipelines for imaging at the highest resolution in tissue and cells. The core has been successful, supporting over 35 NINDS projects across 27 laboratories. For the upcoming grant period, we have expanded the set of microscopes available and have reorganized and simplified the administrative and technical support structure to increase efficiency. Beyond its research mission, the Center will also continue to serve as a nexus for collaborative interactions across neuroscience communities and an educational hub for learning about imaging technologies and approaches. Through the sustained development of this Center, we hope to continue to provide cost-effective and productive services to NINDS researchers while further deepening ties between these two vibrant neuroscience communities.