Macromolecular X-ray Diffraction Core Research Facility

CBSD大分子X射线衍射核心（MXDC）提供支持、专业知识和基础设施， CBSD COBRE研究人员和蒙大拿大学实验室使用X射线晶体学作为工具， 确定小分子和生物大分子的三维结构，并寻求 表达和纯化蛋白质和RNA，用于生物化学、生物物理和结构研究。MXDC 拥有基础设施和专业知识，为用户提供全面的共享主要 在细菌和昆虫细胞中表达重组蛋白的仪器和研究培训，机器人， 高分子的辅助结晶、结晶实验的评价和 小分子和大分子晶体的衍射数据及其三维结构的测定 原子或近原子分辨率的结构。该设施设有理学007 HF X射线发生器和R轴 用于大分子晶体学成像板检测器和Bruker D8 Venture固定Chi双源 用于小分子应用的衍射仪。这两种仪器都具有牛津液氮晶体低温， 冷却系统MXDC常规测量晶体在同步加速器源的X射线衍射数据， 单波长和多波长，用于通过分子置换、重原子和 反常色散定相MXDC的计算资源也可用于X射线数据 小分子和大分子的加工、结构测定、精制和可视化 结构测定MXDC鼓励合作模式，其中教师，学生和工作人员 在核心人员的协助和培训下开展项目。MXDC是一种培训资源， 在蛋白质表达/纯化以及小分子和 大分子X射线晶体学技术。CBSD第二阶段这一组成部分的具体目标 COBRE是：1，通过采用项目程序来监督和维护生产协作设施 规划、适当的费用结构和项目报告机制，以及短期和战略性的 在咨询委员会的指导下进行规划; 2、保持和加强基因到结构和小 通过实施最先进的技术提供分子结构测定服务; 3，进行研究 与CBSD COBRE研究人员在涉及MXDC领域的项目方面进行合作 专业知识;和4，提供蛋白质表达，纯化， 为CBSD研究人员和UM提供结晶和X射线结构测定（大分子和小分子） 教师通过直接合作，研讨会，讲座，MXDC网站和MDXC核心奖学金 程序. 1

The CBSD Macromolecular X-ray Diffraction Core (MXDC) provides support, expertise, and infrastructure to CBSD COBRE Investigators and University of Montana laboratories that use X-ray crystallography as a tool to determine the three-dimensional structures of small molecules and biological macromolecules, and seek to express and purify proteins and RNA for biochemical, biophysical and structural studies. The MXDC possesses the infrastructure and expertise to provide comprehensive user access to shared major instrumentation and research training for expression of recombinant proteins in bacteria and insect cells, robot- assisted crystallization of macromolecules, evaluation of crystallization experiments, and measurement of diffraction data from crystals of both small and macromolecules and determination of their three-dimensional structures at atomic or near-atomic resolution. The facility houses a Rigaku 007HF X-ray generator and R-axis image plate detector for macromolecular crystallography and a Bruker D8 Venture fixed Chi dual source diffractometer for small molecule applications. Both instruments feature Oxford liquid-nitrogen crystal cryo- cooling-systems. The MXDC routinely measures X-ray diffraction data from crystals at synchrotron sources at single and multiple wavelengths for structure determination by molecular replacement, heavy atom, and anomalous dispersion phasing. Computational resources of the MXDC are also available for X-ray data processing, structure determination, refinement, and visualization for both small-molecule and macromolecular structure determination. The MXDC encourages a collaborative model, in which faculty, students and staff conduct projects with assistance and training from Core personnel. The MXDC is a resource for training, education, consultation and collaboration in protein expression/purification and both small molecule and macromolecular X-ray crystallographic techniques. The specific aims of this component of the Phase II CBSD COBRE are: 1, to oversee and maintain a productive collaborative facility by adopting procedures for project planning, an appropriate fee structure and project reporting mechanism, and both short-term and strategic planning with the guidance of an Advisory Committee; 2, to maintain and enhance gene-to-structure and small molecule structure determination services by implementing state-of-the-art technologies; 3, to pursue research collaborations with CBSD COBRE investigators in aspects of their projects that involve areas of MXDC expertise; and 4, to provide training in practical application of techniques in protein expression, purification, crystallization, and X-ray structure determination (macro and small-molecule) for CBSD investigators and UM faculty through direct collaborations, workshops, lectures, the MXDC website and the MDXC Core Fellowship Program. 1