A Novel Cryopreservation Technology for Large Skin Grafts to Facilitate Tissue Banking and Allograft Transplantations

总结 由于免疫遗传学、人工智能（AI）和大规模免疫学的最近和快速发展， 数据技术，供体-受体免疫匹配的准确性和效率， 预计会有很大的改善。因此，传统的组织库实践正在被 由于低温保存通量低而受到严重挑战。同种异体皮肤移植 目前用于许多临床应用。在这种应用中，供体组织现在 通常用作“昂贵的绷带”，即用于临时覆盖，因此需要疼痛 和破坏后续自体移植程序。有了极大的改进皮肤移植 冷冻保存方法结合使用基于AI网络的HLA配型，未来组织 银行平台有可能提供移植物，作为永久性和确定性的治疗方法， 从而对临床应用和医疗保健行业产生重大影响。 基于CryoCrate的新型超快（样品表面106 K/min）冷却技术 平台（PCT/US 2019/26162），该项目旨在开发一种高效（接近100%）的 解冻后存活力）组织移植物冷冻保存原型系统，其不需要冷冻保护剂。 解冻后活力和功能将通过标准体外试验使用猪 和人皮肤（研究豁免）移植物以及使用小鼠模型的体内测定。后 为了实现这些目标，在未来的第二阶段和第三阶段，CryoCrate及其学术合作者 将与当地器官采购组织（中美洲移植）和 伤口愈合诊所（密苏里州大学医院），用于猪和人皮肤移植 研究和临床试验。

SUMMARY As a result of recent and rapid advances of immunogenetical, artificial intelligence (AI) and big data technologies, the accuracy and efficiency of donor-recipient immunogenetical matching are expected to be significantly improved. As such, traditional tissue banking practices are being severely challenged due to low cryopreservation throughput. Skin allograft transplantations are currently utilized for numerous clinical applications. In such applications, donor tissues are now typically utilized as “expensive bandages”, i.e. for temporary coverage, thereby requiring painful and damaging follow-on autotransplantation procedures. With tremendously improved skin graft cryopreservation methods coupled with use of HLA matching based on AI network, future tissue banking platforms can potentially provide grafts to be used as permanent and definitive treatment for patients, thereby bringing high impact to clinical applications and the healthcare industry. Based on CryoCrate’s novel ultra-fast (106 K/min on a sample surface) cooling technology platform (PCT/US2019/26162), this project aims to develop a highly efficient (approaching 100% post-thaw viability) tissue graft cryopreservation prototype system that requires no cryoprotectant. The post-thaw viability and functionality will be assessed by standard in vitro assays using porcine and human skin (research exempt) grafts and in vivo assays using a mouse model. After achieving these aims, in future phase II and III stages, CryoCrate and its academic collaborator will collaborate with a local Organ Procurement Organization (Mid-America Transplant) and a wound healing clinic (University of Missouri Hospital) for porcine and human skin transplantation studies and clinical trials.