A Comprehensive Functional Map of Human Protein-RNA Interactions

资源项目--项目摘要 Encore(RNA元素百科全书)的总体目标是提供蛋白质-RNA相互作用图 2个人类细胞系中的300个限制性商业惯例，以及相关的定位、结合基序和功能数据集，有助于 对这些数据进行解释，并为大部分限制性商业惯例分配职能。这个资源项目的目标是 是建立和验证稳定表达表位标记的RNA结合蛋白的细胞系，进行初步 和对人类RBP物理和功能相互作用数据的综合分析，并公开开发- 可访问的转录、结合亲和力和亚细胞定位数据库。这三个目标至关重要 通过不仅为社区提供宝贵的物质资源来最大化这种资源的影响 (例如标记的细胞系)，但也集成了数据分析，以获得尽可能多的洞察 每个RBP的功能和结合特性。更重要的是，让这些数据尽可能地被 通过在公共数据门户上提供所有数据，如 编码DCC，以及通过我们自己的门户网站，这些门户网站以符合基于Web的格式托管数据集 查询和重点浏览。我们还将举办有关使用这些工具的培训课程。最后，我们会 通过使用通用工作流语言使我们开发的所有代码可用来促进可重现的数据分析 可以在任何计算服务器和平台上使用。

RESOURCE PROJECT - PROJECT SUMMARY The overall goal of ENCORE (Encyclopedia of RNA Elements) is to provide protein-RNA interaction maps for 300 RBPs in 2 human cell lines, and associated localization, binding motif and functional datasets that aid in interpretation of these data and assigning functions to the bulk of these RBPs. The goal of this resource project is to generate and validate stable cell lines expressing epitope-tagged RNA binding proteins, perform primary and integrated analysis of the human RBP physical and functional interaction data, and to develop publicly- accessible transcriptomic, binding affinity and subcellular localization databases. These three goals are critical to maximizing the impact of this resource by providing not only valuable physical resources to the community (e.g. tagged cell lines) but also integrated analysis of the data to obtain as much insight as possible into the functional and binding properties of each RBP. More importantly, making this data as accessible as possible to a broad community of scientists will be achieved by making all data available at public data portals such as the ENCODE DCC, as well as via our own web portals that host the datasets in formats amenable for web-based queries and focused browsing. We will also conduct training sessions in the use of these tools. Finally, we will facilitate reproducible data analysis by making all code we develop available using common workflow language that can be used on any compute server and platform.