Live cell, high speed and resolution, spectral confocal microscope

描述(由申请人提供)：这份申请来自马萨诸塞州伍兹霍尔的海洋生物实验室(MBL)，请求为购买高端共聚焦显微镜提供资金。我们介绍了蔡司LSM-710 NLO/LSM Live Duo的案例。拟议的仪器将配备一系列配件，以满足我们用户群体的需求--特别是我们的主要用户。LSM-710将取代2002年购买的中央显微镜设备(CMF)中的蔡司LSM-510。新系统利用了激光、探测器和软件组件的最新进展。例如，光谱成像的完成速度比LSM-510快得多。灵敏度和信噪比也得到了提高。简而言之，波音710在很多方面都更高效。我们进一步提出了710相对于竞争对手的仪器具有显著的优势，并对这些优势进行了详细的讨论。然而，认识到这是一个快速变化的领域，如果获得这笔赠款，我们将重新评估重大变化的情况。我们提出的大部分研究都集中在活细胞成像和细胞动力学上。提交的研究项目被细分为四个小组。第一个包括NIH资助的居民和访问研究，并包括我们的5个主要用户项目。这些重大项目中有四个是由研究人员支持的，他们总共持有9名相关的NIH个人研究员补助金(1名功勋奖和8名R01)。常驻研究包括来自伍兹霍尔联盟内两个研究所的申请；海洋生物 实验室(MBL)和伍兹霍尔海洋研究所(WHOI)。这两家公司都进行了广泛的研究，具有强大的生物医学成分。这第一类访问研究涵盖了MBL惠特曼研究人员和MBL细胞动力学计划的兼职成员。首席调查员指导后者。我们在这第一类中包括11个项目。我们的第二个用户类别包括由其他联邦机构资助的调查人员的申请(4个项目：NSF和ONR)。我们的第三类包括非联邦资助的访问调查人员(2个项目)。最后，在第4类中，我们简要介绍了在博士后到教授阶段开设的5门相关的MBL课程，这些课程将利用新的工具。其中两项也得到了美国国立卫生研究院的拨款支持。蔡司710将被安置在CMF中，CMF在维护高端仪器、向广泛的用户提供它以及在仪器的最佳使用方面培训研究人员方面有着典范的历史。长期以来，在MBL安装仪器的商业实体一直提供支持，尤其是Carl Zeiss Inc.的这一应用。较新设计的效率产生了几个环境优势，许多工作与购买这种高端仪器密切相关。对这些问题进行了详细的讨论。

DESCRIPTION (provided by applicant): This application, from the Marine Biological Laboratory (MBL), Woods Hole, MA, requests funding for the purchase of a high end confocal microscope. We present our case for the Zeiss LSM-710 NLO/LSM Live DUO. The proposed instrument will be equipped with an array of accessories dictated by the needs of our user group - in particular our major users. The LSM-710 will replace a Zeiss LSM-510 in the Central Microscopy Facility (CMF) that was purchased in 2002. The new system takes advantage of recent advancements in lasers, detectors and software components. Spectral imaging, for example, is accomplished at a much faster rate than the LSM-510. Sensitivity and signal-to-noise are also improved. The 710 is, in short, more efficient in many ways. We further propose that the 710 has significant advantages over competing instruments and discuss these in detail. However, recognizing that this is a rapidly changing field we would reappraise the situation for significant changes if awarded this grant. The majority of our proposed research focuses on live cell imaging and cell dynamics. The submitted research projects are subdivided into four groups. The first comprises NIH funded resident and visiting re- search and include our 5 major user projects. Four of these major projects are supported by researchers' holding a total of nine relevant individual investigator NIH grants (1 Merit Award and 8 R01s). Resident research includes applications from two of the institutes within the Woods Hole Consortium; the Marine Biological Laboratory (MBL) and the Woods Hole Oceanographic Institution (WHOI). Both undertake a broad range of re- search, with strong biomedical components. Visiting research in this first category covers MBL Whitman investigators and adjunct members of the MBL Cellular Dynamics Program. The Principal Investigator directs the latter. We include 11 projects in this first category. Our second user category includes applications from investigators funded by other federal agencies (4 projects: NSF and ONR). Our third category includes visiting investigators with non-federal funding (2 projects). Lastly, in category 4, we include short descriptions for 5 relevant MBL courses, offered at the post-doctoral to professorial level, which would make use of the new instrument. Two of these are also supported by NIH grants. The Zeiss 710 would be housed in the CMF, which has an exemplary history of maintaining high end instrumentation, making it available to a broad spectrum of users, and training researchers in the instrument's optimal use. There is a long history of support from commercial entities installing instrumentation at the MBL, notably for this application from Carl Zeiss Inc. The efficiency of the newer design yields several environmental advantages and a number of jobs are intimately connected with the purchase of this high end instrument. These are discussed in detail.