NARMS Retail Food Surveillance in Ohio

摘要 抗菌素耐药性是对人类和动物健康日益严重的威胁。在美国 仅每年就有23,000人死于抗菌素耐药性感染 有机体。接触耐药细菌病原体的一个常见来源是食物，尤其是新鲜食物。 零售肉类产品。零售肉制品中存在的大多数食源性致病菌也 在生产期间对食用动物的胃肠道进行定植。肉类的污染 产品发生在屠宰过程中，当GI含量，包括粪便细菌， 污染身体。尽管食品安全有所改善，但食源性疾病的负担 在过去十年中保持相对不变，每年影响美国六分之一的人。 为了帮助解决这一重要的公共卫生问题，我们建议完成以下工作 具体目标：1)促进食源性病原体和指标的国家监测 细菌作为NARMS零售食品监测计划的一部分，2)支持疫情 通过提供现场流行病学和实验室支持来调查食源性疾病 俄亥俄州的其他机构，以及3)进行实地研究，以了解人畜共患病的宿主 食源性传播与耐药病原菌的分子流行病学 以及新鲜零售肉制品中的指示性细菌。 为了实现这些具体目标，我们将作为国家肉类和海鲜零售监测机构 项目贡献实验室。作为一个大型聚集性监测点，我们将对零售家禽进行抽样， 来自俄亥俄州大都市和乡村零售店的牛肉、猪肉和海鲜。四十八岁 每月的样本将进行特定病原体和指示性细菌的培养。由此产生的 分离的菌株将进一步鉴定，并每月运往FDA进行抗菌药敏感性检测 测试，在我们的培养库中保留了重复的分离株。我们期待着成功 每年抽检528包零售牛肉、猪肉、家禽和海鲜，这将有助于跟踪 食源性致病菌和指示菌的耐药性现状 在零售肉类和海鲜中发现，但也将影响未来FDA的政策和法规 监督行动和干预措施的成功。除了我们对零售业的贡献 食品监测计划，作为NARMS的参与实验室，我们将提供支持 根据需要在俄亥俄州和附近各州进行食源性疾病暴发调查。

Summary Antimicrobial resistance is an increasingly critical threat to human and animal health. In the US alone, >23,000 individuals die each year because of infections with antimicrobial resistant organisms. A common source of resistant bacterial pathogen exposure is food, in particular fresh retail meat products. Most foodborne bacterial pathogens present in retail meat products also colonize the gastrointestinal tract of food animals during production. Contamination of meat products occurs during the slaughter process when GI contents, including fecal bacteria, contaminate the carcass. Despite improved food safety, the burden of foodborne illness has remained relatively unchanged for the past decade, affecting 1 in 6 individuals in the US annually. To help address this important public health problem, we propose to accomplish the following specific aims: 1) Contribute to national surveillance of foodborne pathogens and indicator bacteria as part of the NARMS retail food surveillance program, 2) Support outbreak investigations of foodborne disease by providing field epidemiology and laboratory support to other agencies in Ohio, and 3) Conduct field research to understand the reservoirs for zoonotic foodborne transmission, and the molecular epidemiology of antimicrobial resistant pathogens and indicator bacteria in fresh retail meat products. To accomplish these specific aims, we will serve as a NARMS retail meat and seafood surveillance project contributing laboratory. As a large cluster surveillance site, we will sample retail poultry, beef, pork, and seafood from retail stores within both metropolitan and rural Ohio. Forty-eight monthly samples will be cultured for specific pathogens and indicator bacteria. The resulting isolates will be further characterized and shipped monthly to FDA for antimicrobial susceptibility testing, with duplicate isolates maintained in our culture collection. We expect to successfully sample 528 packages of retail beef, pork, poultry, and seafood annually that will help to track the current state of antimicrobial resistance among foodborne pathogens and indicator bacteria found in retail meat and seafood but will also impact future FDA policy and regulations and monitor the success of actions and interventions. In addition to our contributions to the retail food surveillance program, as a NARMS participating laboratory we will be available to support foodborne disease outbreak investigations in Ohio and nearby states as needed.