Project 1: Microbiology of ME/CFS

项目1 微生物学ME/CFS 摘要 许多肌痛性脑脊髓炎/慢性疲劳综合征（ME/CFS）患者报告前驱症状 与感染和/或炎症一致据报道，高达40%的患者对静脉注射 输注Toll样受体3激动剂Ampligen，一种双链RNA类似物， 病毒复制其他人报告了对疱疹病毒特异性抗病毒药物、益生菌和益生菌的反应， 粪便移植，或消耗B细胞的单克隆抗体。我们假设至少有一些 ME/CFS患者的疾病具有感染性触发因素，并且未能涉及感染因子 反映了采样不充分和/或分析不适当。探讨感染和免疫在 ME/CFS，我们将利用敏感的基于序列的方法来检测和表征细菌， 病毒和真菌，使用血液，口腔和粪便样本充分表征ME/CFS病例和对照。 该项目有可能导致基于生态失调的动物模型的发展， 确定可能从抗病毒、抗生素或益生菌干预中获益的ME/CFS患者。

Project 1 Microbiology of ME/CFS Abstract Many patients with Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) report a prodrome consistent with infection and/or inflammation. Up to 40% of patients are reported to respond to intravenous infusions of the Toll-like receptor 3 agonist, Ampligen, a double-stranded RNA analogue proposed to inhibit viral replication. Others report responses to antiviral drugs specific for herpesviruses, pre- and probiotics or fecal transplantation, or monoclonal antibodies that deplete B cells. We hypothesize that at least some ME/CFS patients have an infectious trigger for their disease, and that failures to implicate infectious agents reflect inadequate sampling and/or inappropriate assays. To explore the role of infection and immunity in ME/CFS, we will exploit sensitive sequence-based methods for detection and characterization of bacteria, viruses, and fungi, using blood, oral, and fecal samples from well characterized ME/CFS cases and controls. This project has the potential to lead to the development of animal models based on dysbiosis as well as the identification of patients with ME/CFS who may benefit from antiviral, antibiotic, or probiotic interventions.