Scale up single-cell technologies to map pain-associated genes and cells across the lifespan

项目总结 慢性疼痛是复杂的，多样的，难以管理的，而目前的治疗方法，如阿片类药物，不是 对许多人都有效。调节慢性疼痛的基因、回路和细胞仍然是一个黑匣子。在……里面 特别是，研究调节慢性疼痛的基因、电路和细胞如何在人的一生中发生变化 弱势群体，包括婴儿、儿童、老年人、孕妇，研究不足，往往被忽视。 在了解慢性疼痛的潜在机制方面的知识差距主要是由于缺乏 以高分辨率和高分辨率绘制和解码疼痛相关基因、电路和细胞的创新技术 比例。我们将开发高度模块化和广泛性的新型单细胞技术“Raman2Omics” 适用于绘制与疼痛相关的基因、电路和细胞图。我们将利用一个小鼠模型 术后疼痛研究不同年龄、性别和年龄的潜在分子机制 怀孕了。我们将应用“Raman2Omics”系统地研究与之相关的基因和细胞 术后疼痛程度较重。有了这些创新的单电池技术，我们将能够产生高度 有价值的分子信息和确定新的候选生物标记物和慢性疼痛的治疗靶点， 这将导致新的诊断和治疗解决方案的开发。同时，“Raman2Omics”可以 可方便地应用于健康和疾病中的其他多细胞复杂组织的研究。

PROJECT SUMMARY Chronic pain is complex, diverse, and difficult to manage, and current treatments, such as opioids, are not effective for many individuals. The genes, circuits, and cells regulating chronic pain remain a black box. In particular, research on how the genes, circuits, and cells regulating chronic pain change across the lifespan in vulnerable populations including infants, children, elders, pregnant women is understudied and often ignored. The knowledge gap in understanding the underlying mechanisms of chronic pain is mainly due to the lack of innovative technologies to map and decode the pain-associated genes, circuits, and cells at high resolution and scale. We will develop novel single-cell technologies “Raman2Omics” that are highly modular and broadly applicable to map the genes, circuits, and cells associated with pain. We will utilize a mouse model of postoperative pain to investigate the underlying molecular mechanisms across ages, sexes, and during pregnancy. We will apply “Raman2Omics” to systematically investigate genes and cells associated with postoperative pain at scale. With these innovative single-cell technologies, we will be able to generate highly valuable molecular information and identify novel candidate biomarkers and therapeutic targets for chronic pain, which will lead to the development of novel diagnosis and treatment solutions. Meanwhile, “Raman2Omics” can be readily applied to investigate other multicellular complex tissues in health and diseases.