Molecular Biology and Immunopathology Core

分子生物学概述免疫病理学核心。 路易斯安那州立大学(LSU)拟建的临床前癌症研究中心 专注于利用专门的疾病模型阐明人类癌症的分子和细胞机制 它们是人类疾病的预兆。癌症中心将专注于具有破坏性的人类癌症 需要新的治疗策略的患者的治疗结果不佳。由Highly牵头的拟议项目 有前途的初级调查人员(PJI)需要先进的分子生物学和 免疫病理学原理、方法，特别是基因组学、转录组学和蛋白质组学的进展， 免疫学和病理学，以描述每一种特定癌症的关键细胞机制 最先进的设备和技术专长。分子生物学和免疫病理学的核心 (MBIPC)是为了利用和扩大生物技术司的资源和专门知识而设立的 和路易斯安那州立大学兽医学院的分子医学(BioMMED)，该学院管理着六个 核心设施：基因表达和测序实验室(GenLab)、病毒载体实验室、蛋白质 鉴定和纯化实验室(PCPL)，该实验室还产生单特异性和单克隆性 抗感兴趣蛋白质抗体，小动物成像实验室(SAIL)和分子 提供免疫病理学专业知识并访问支持向量机的免疫病理实验室(MIPL) 集中化设施(FACS和病理成本中心)，还提供专业知识和访问生物成像 以及先进的免疫荧光和电子显微镜。这些实验室目前由 路易斯安那州生物医学研究网络(LBRN/INBRE)，它还运营着LBRN生物信息学核心 与路易斯安那州立大学计算与技术中心(CCT)密切合作。MBIPC的目标是提供 并协调获得先进仪器设备和服务的状态，并提供广泛的培训 所有PJI和其他NIH的基础和高级分子生物学和免疫病理学的光谱- 资助研究人员实现他们的研究目标。MBIPC确定了两个具体目标： 目标1.为癌症研究中心和其他由NIH资助的研究人员提供资源和专业知识 分子和细胞生物学和免疫病理学，包括仪器、技术支持、咨询和 培训，并协助开发新的方法。 目标2：组织、协调和促进Cobre调查人员之间的互动和协作 开发和使用新技术和分析方法，并对PJI、教职员工和学生进行高级培训 分子生物学和免疫病理学技术。

Summary Molecular Biology Immunopathology Core. The proposed Center for Pre-Clinical Cancer Research (Cancer COBRE) at Louisiana State University (LSU) focuses on elucidating molecular and cellular mechanisms of human cancer using specialized disease models that are predictive of disease in humans. The Cancer COBRE will focus on devastating human cancers that have poor treatment outcome in patients in need of novel therapeutic strategies. The proposed projects led by highly Promising Junior Investigators (PJIs) require the application of advanced molecular biological and immunopathological principles, methods and particularly advances in genomics, transcriptomics and proteomics, immunology and pathology to delineate key cellular mechanisms for each specific cancer necessitating access to state-of-the-art equipment and technical expertise. The Molecular Biology and Immunopathology Core (MBIPC) has been created to leverage and expand the resources and expertise of the Division of Biotechnology and Molecular Medicine (BioMMED) at the LSU School of Veterinary Medicine (SVM), which administers six Core Facilities: Gene Expression and Sequencing Laboratory (GeneLab), Viral Vectors Laboratory, Protein Characterization and Purification laboratory (PCPL), which is also generates monospecific and monoclonal antibodies to proteins of interest, the Small Animal Imaging Laboratory (SAIL) and the Molecular Immunopathology laboratory (MIPL) that provides expertise in immunopathology and access to the SVM Centralized Facilities (FACS and Pathology Cost Centers), and also provides expertise and access to bioimaging and advanced immunofluorescence and electron microscopy. These laboratories are currently co-supported by the Louisiana Biomedical Research Network (LBRN/INBRE), which also operates the LBRN Bioinformatics Core in close collaboration with the LSU Center for Computation & Technology (CCT). The goal of MBIPC is to provide and coordinate access to state of the advanced instrumentation and services and provide training in a broad spectrum of fundamental and advanced molecular biology and immunopathology to all PJIs and other NIH- funded investigators to achieve their research objectives. MBIPC has identified two specific aims: Aim 1. To provide the Cancer COBRE and other NIH-funded investigators with resources and expertise in molecular and cell biology and immunopathology including instrumentation, technical support, consultation and training, and assistance in developing new methodologies. Aim 2: To organize, coordinate, and facilitate interactions among COBRE investigators and work collaboratively to develop and use new technologies and assays, and train PJIs, faculty, staff, and students in advanced molecular biology and immunopathology technologies.