CSHL Advanced Techniques in Molecular Neuroscience Course

摘要 CSHL分子神经科学高级技术实验室课程 建议开设的冷泉港实验室(CSHL)分子高级技术课程 神经科学(ATMN)将于2020-2024年7月举行，是CSHL研究生的一部分 神经生物学培训计划。ATMN是一门为期16天的短期强化实践课程， 使学生做好准备，直接进入利用高级和/或专业的研究 当代神经生物学研究中的分子技术。中的实验模块 这门课程将包括定量分析通过交联结合到蛋白质上的RNA。 免疫沉淀法(CLIP)，翻译从特定神经亚型(TRAP)、组织中分离的mRNA 结合杂交链式反应检测单个RNA分子的清除法 (Hcr)，纯星形胶质细胞和神经前体细胞培养的制备，工程化的设计和使用 病毒载体在神经科学研究中的应用CRISPR/CAS基因组编辑工具的设计和使用，实用 体内基因传递系统的练习，染色质状态的全基因组评估和 ATACseq和Cut&Run结合转录因子及BAC转基因的应用与设计 向量。实验技术是在更广泛的概念进步的背景下教授的， 他们可能会提供。本课程邀请在各自领域做出重大贡献的讲师 放弃关于当前研究的最新报告。受训人员由课程教员从 申请者人数更多，范围从研究生到高级调查人员和研究人员 医生。由于课程时间较短，高年级学生和低年级学生都可以参加。 并在远离他们的其他需求的环境中接受短期、高强度的训练 时间和注意力。ATMN课程也为知名科学家提供了一个不同寻常的机会 将这些先进技术应用于自己的研究兴趣，或在重要专业上进行再培训 在他们可能不熟悉的神经科学中。本课程讲授的方法和概念 通过出版实验室手册和向更广泛的研究社区传播 在线资源。

ABSTRACT CSHL Laboratory Course in Advanced Techniques in Molecular Neuroscience The proposed Cold Spring Harbor Laboratory (CSHL) course Advanced Techniques in Molecular Neuroscience (ATMN), to be held annually in July 2020– 2024, is part of the CSHL's postgraduate training program in neurobiology. ATMN is a short, intensive sixteen day hands-on course which prepares students to enter directly into research that makes use of advanced and/or specialized molecular techniques in contemporary aspects of neurobiological research. Experimental modules in the course will include quantitative assays of RNAs bound to protein by crosslinking immunoprecipitation (CLIP), translating mRNA isolation from specified neural subtypes (TRAP), tissue clearing methodologies combined with single RNA molecule detection by hybridization chain reaction (HCR), preparation of pure astrocyte and neural progenitor cell cultures, design and use of engineered viral vectors in neuroscience research, design and use of CRISPR/Cas genome editing tools, practical exercises in gene delivery systems in vivo, genome-wide assessment of chromatin state and transcription factor binding by ATACseq and Cut & Run, and use and design of BAC transgenic vectors. Experimental techniques are taught in the context of the broader conceptual advances that they may provide. The course invites lecturers who have made significant contributions in their fields to give up-to-the-minute reports on current research. The trainees are chosen by the course faculty from larger pools of applicants and range from graduate students to senior investigators and research physicians. Because of the short duration of this course, senior, as well as junior individuals can attend and receive a short, intense period of training in an environment remote from other demands on their time and attention. The ATMN course also provides an unusual opportunity for established scientists to apply these advanced techniques to their own research interests, or to retrain in an important specialty within neuroscience with which they may not be familiar. Methods and concepts taught in the course are disseminated to the wider research community through the publication of laboratory manuals and online resources.