Biomedical Engineering Core

摘要 在可获取的样本(血液、唾液、尿液等)中发现循环标志物代表了一个令人兴奋的生物标记物 类，因为保护它们具有微创的性质。潜在地，这些循环标记可以使 旨在了解一种疾病的病理生理学并将这些发现转化为 通过匹配疾病的分子特征来管理人类疾病宿主的床边 到适当的治疗方案(即精准医学)。循环记号笔(即液体)的吸引力 活检标记物)是样本中发现的过多的标记物类型，如生物细胞，无细胞 分子(蛋白质和无细胞DNA)和细胞外小泡(如外切体等纳米组件)。 不幸的是，这些液体活检标记物的基础研究和临床翻译一直具有挑战性，因为 缺乏有效的分离平台，也可以容纳下游分子 流通标记货物的特性。KIPM将生成生物医学工程核心，将 向Cobre调查人员提供转型工具，包括硬件和相关分析，这些工具可以 为手头的项目编制程序，并向调查人员提供高质量的循环标记作为 各种分子特征分析(DNA/RNA下一代测序、蛋白质组学、 免疫分析、突变检测、液相色谱/质谱仪等)。这个 硬件工具是经过优化的芯片实验室或微流控平台，用于隔离 循环标志物，并在各种应用领域得到临床验证。微流控工具还具有 经过验证的分析和由BME核心成员开发的自动化工作流程。这些 与市场上针对 循环标志物的分离。以低成本、大规模生产的方式生产微流体 因为它们是由塑料制成的，并通过注射成型形成适当的结构，因此 建立了生产CD、DVD和蓝光光盘的生产线。使用这些工具集，BME CORE将创建一个实验室，可以立即为Cobre调查人员的精确医学提供服务 基于项目的。由于BME核心使用的工具的独特功能，生成的数据集 KIPM调查人员和获取这些数据集的独特工具将提高他们在 为他们的项目寻求联邦资金支持。对于无法通过以下方式有效服务的Cobre项目 核心的现有工具，核心将与项目PI合作，设计新的工具，以适应他们的 该项目还将向BME核心添加新的流程功能。BME核心具有分析设计 支持这一活动的能力和原型工具。

Abstract Circulating markers found in accessible samples (blood, saliva, urine, etc.) represent an exciting biomarker class due to the minimally invasive nature of securing them. Potentially, these circulating markers can enable studies directed toward understanding the pathophysiology of a disease and translating those discoveries to the bedside for managing a host of human diseases by matching the molecular characteristics of the disease to proper treatment regimens (i.e., precision medicine). The attractive nature of circulating markers (i.e., liquid biopsy markers) is the plethora of marker types found in the sample such as biological cells, cell-free molecules (proteins and cell-free DNA) and extracellular vesicles (nanometer assemblies such as exosomes). Unfortunately, basic studies and clinical translation of these liquid biopsy markers has been challenging due to the lack of efficient platforms for their isolation that can also accommodate downstream molecular characterization of the circulating marker cargo. KIPM will generate the Biomedical Engineering Core that will provide to COBRE investigators transformative tools, including hardware and the associated assays, that can be programmed for the project at hand and provide to investigators high quality circulating markers to serve as inputs for a variety of molecular characterization assays (DNA/RNA Next Generation Sequencing, proteomics, immunoassays, mutation detection, liquid chromatography/mass spectrometry, and many others). The hardware tools are lab-on-a-chip or microfluidic platforms that have been optimized for the isolation of circulating markers and clinically validated in a variety of application areas. The microfluidic tools also have validated assays and an automated workflow that has been developed by members of the BME Core. These tools have noteworthy performance metrics compared to commercially available products directed for the isolation of circulating markers. The microfluidics are produced in a high scale production mode at low cost because they are made from plastics and formed into the appropriate structures using injection molding, an established production pipeline for producing CDs, DVDs and Blu-Ray discs. Using these tool sets, the BME Core will create a laboratory that can immediately service COBRE investigators on their precision medicine based projects. Due to the unique capabilities of the tools employed by the BME Core, the data sets generated by the KIPM investigators and the unique tools to acquire these data sets will improve their competitiveness in seeking federally-funded support of their projects. For COBRE projects that cannot be effectively serviced by the Core’s existing tools, the Core will work with the project PI to design new tools to accommodate their project that will also add new process capabilities to the BME Core. The BME Core has assay design capabilities and prototyping tools to support this activity.