Mouse Metabolic Physiology Core

项目摘要 小鼠代谢生理学核心及其两个子核心，代谢调节子核心和身体 体重调节子核心，为研究人员提供实验设计和使用状态的指导， 评估胰岛素作用，底物代谢， 和体内能量平衡。这个核心的经验丰富的教师和高技能的工作人员有20年的历史 进行复杂的程序，以研究健康，无压力的小鼠的代谢，并已在 新概念和新技术的开发、标准化、实施和传播的前沿 来研究代谢性疾病的小鼠模型。在本申请中，小鼠代谢生理学 核心提供急需的鼠标相关的服务，以调查人员以外的范德比尔特。巧鼠 外科医生进行诸如导管、脑插管和脑探头植入等困难的手术， 减肥手术和胰岛移植。经验丰富的工作人员进行复杂的实验，如代谢 在诸如由葡萄糖钳夹或运动产生的条件下的通量分析。能量测量 平衡成分和奖励/动机行为的评估允许生理和行为 在代谢疾病的小鼠模型中确定体重的决定因素。核心合并 技术，以便氧摄取，二氧化碳输出，能量消耗，活动和进食行为， 与使用留置导管进行的代谢研究或使用 植入套管和光纤探针。范德比尔特过去5年的经验表明， 这个核心。小鼠代谢生理学核心参与重要的教育计划， 糖尿病社区，包括为期一周的课程，19年来已经进行了20次，重点是 在小鼠中进行葡萄糖钳夹所必需的外科手术和实验技术。此核心支持 范德比尔特充满活力的计划，提供必要的时间和资源，以调查人员从代表不足 人口或机构和早期职业调查人员。总之，核心促进糖尿病，肥胖， 和代谢研究，提供新的服务，是可行的，在少数其他机构的调查 就在范德比尔特外面

Project Summary The Mouse Metabolic Physiology Core and its two subcores, the Metabolic Regulation Subcore and the Body Weight Regulation Subcore, provide investigators both guidance on experimental design and use of state-of- the-art techniques to assess genetic and environmental determinants of insulin action, substrate metabolism, and energy balance in vivo. The experienced faculty and highly skilled staff of this Core have a 20-year history of performing complex procedures to study metabolism in healthy, unstressed mice and have been at the forefront of development, standardization, implementation, and dissemination of new concepts and techniques to study mouse models of metabolic diseases. In the present application, the Mouse Metabolic Physiology Core delivers critically needed mouse-related services to investigators outside of Vanderbilt. Skilled mouse surgeons perform difficult surgeries such as catheter, brain cannula, and brain probe implantations, as well as bariatric surgery and islet transplantation. Experienced staff perform complex experiments such as metabolic flux analyses under conditions such as those created by a glucose clamp or exercise. Measurements of energy balance components and assessment of reward/motivated behavior allow for the physiological and behavioral determinants of body weight to be determined in mouse models of metabolic disease. This Core merges techniques so that oxygen uptake, carbon dioxide output, energy expenditure, activity and feeding behavior are measured simultaneously with studies of metabolism using indwelling catheters or neural regulation using implanted cannula and fiber optic probes. Experience at Vanderbilt over the past 5 years predicts robust use of this Core. The Mouse Metabolic Physiology Core participates in important educational programs for the diabetes community, including a weeklong course that has been given 20 times over 19 years focusing on surgical and experimental techniques necessary to perform glucose clamps in mice. This Core supports the Vanderbilt Vibrant Program by providing necessary time and resources to investigators from underrepresented populations or institutions and early-career investigators. In summary, the Core facilitates diabetes, obesity, and metabolism research by providing novel services that are feasible at few other institutions to investigators outside of Vanderbilt.