Cellular Senescence and Beyond Core

摘要 细胞命运是与年龄相关的分子事件之间的重要中间体，包括损伤和 压力，以及作为衰老生物体标志的表型和病理学。细胞命运也能提供 这是对许多与年龄有关的疾病进行干预的独特和广泛适用的机会。的 细胞衰老和超越核心（CSB）在这个内森休克中心（NSC）的应用程序将有助于 学术研究人员确定特定的细胞命运是否以及在多大程度上在其衰老和/或 干预模式。根据项目的不同，核心将表征细胞的自主性和非自主性。 选定的细胞命运的自主特征，告知研究人员它们可能的生理后果， 并测试提出的干预策略。目标1将建立和提供标准化的方法来检测细胞- 细胞衰老、细胞死亡和细胞竞争的细胞命运决定的相关标志物。目标2将 建立标准化方法，以检测和评估细胞非自主效应的影响， 从细胞衰老、细胞死亡和细胞竞争的细胞命运决定。核心将采用 各种技术，包括ELISA、免疫细胞化学、Western分析和特别有用的质谱分析。 光谱法提供全面的，公正的评估。核心还将向调查人员提供以下建议： 识别最有希望的细胞自主和非自主代理候选者的策略， 相关测定以测试这些候选物的生物活性。综上所述，所提出的蜂窝 衰老和超越核心将提供研究人员在老化研究与广泛的实验阵列， 关于如何确定选定的细胞命运决定是否重要以及在多大程度上重要的分析和建议 研究者感兴趣的年龄相关表型和病理学的贡献者。

ABSTRACT Cell fates are important intermediates between age-related molecular events, including damage and stress, and the phenotypes and pathologies that are hallmarks of aging organisms. Cell fates can also offer unique -- and broadly applicable -- opportunities for interventions into many age-related diseases. The Cellular Senescence and Beyond Core (CSB) in this Nathan Shock Center (NSC) application will help academic researchers determine whether and to what extent specific cell fates play a role in their aging and/or intervention models. Depending on the project, the Core will characterize cell autonomous and non- autonomous features of selected cell fates, advise investigators of their possible physiological consequences, and test proposed intervention strategies. Aim 1 will establish and offer standardized methods to detect cell- associated markers of the cell fate decisions of cellular senescence, cell death and cell competition. Aim 2 will establish standardized methods to detect and assess the impact of the cell non-autonomous effects resulting from the cell fate decisions of cellular senescence, cell death and cell competition. The core will employ a variety of techniques, including ELISAs, immunocytochemistry, western analyses and, of particular utility, mass spectrometry to provide comprehensive, unbiased assessments. The Core will also advise investigators on strategies to identify the most promising cell autonomous and non-autonomous acting candidates and perform relevant assays to test these candidates for biological activity. In summary, the proposed Cellular Senescence and Beyond Core will provide investigators in aging research with a broad array of experimental assays and advice on how to determine whether and to what extent selected cell fate decisions are important contributors to the age-related phenotypes and pathologies of interest to the investigators.