Heterogeneity in bacterial quorum sensing during infection

项目总结 细菌必须表达某些因子才能在宿主内成功定植和繁殖。很多因素 被控制在转录水平上。大约60%的细菌基因组编码替代的西格玛 Sigma-54因子，激活启动子的一个子集。依赖Sigma-54的转录启动 启动子需要来自细菌增强结合蛋白催化的三磷酸腺苷的水解能 它结合在启动子的上游。这项建议的总体目标是增加对如何 BEBPs促进最初的宿主-微生物相互作用。为了实现这一目标，编码在 将调查费氏弧菌对其自然宿主结合能力的影响。五. 鱼细胞在某些无脊椎动物的光器官内定植和生长，包括夏威夷短尾鱼 乌贼Euprymna Scolope。对已定义的bEBP突变体的研究工作包括定植分析，这 将决定每种bEBP对费氏弧菌定殖寄主能力的影响程度。 还建议进行转录切割分析，以确定每种bEBP如何影响V。 费尔切里。总而言之，这些结果将决定每个bEBP对费氏拟青霉能力的贡献。 与东道主建立长期的联系。应聘者将接受学士学位毕业后的培训 微生物学研究和职业发展机会，这将为候选人做好准备 微生物学的研究生课程。

PROJECT SUMMARY Bacteria must express certain factors to successfully colonize and proliferate within a host. Many factors are controlled at the level of transcription. Roughly 60% of bacterial genomes encode the alternative sigma factor sigma-54, which activate a subset of promoters. Transcriptional initiation of sigma-54-dependent promoter requires energy from the hydrolysis of ATP, which is catalyzed by a bacterial enhance binding protein that binds upstream of the promoter. The overall goal of this proposal is to increase understanding of how bEBPs promote initial host-microbe interactions. To achieve this goal, the bEBPs encoded within the bacterium Vibrio fischeri will be investigated for their impact on the ability to associated with its natural host. V. fischeri cells colonize and grow within the light organs of certain invertebrates including the Hawaiian bobtail squid Euprymna scolopes. Research efforts with defined bEBP mutants include colonization analyses, which will determine the extent to which each bEBP impacts the ability of V. fischeri to colonize its host. Transcriptomic analyses are also proposed to determine how each bEBP affects cellular physiology of V. fischeri. Together, these results will determine the contribution of each bEBP to the ability of V. fischeri to establish a long-term association with its host. The candidate will receive post-baccalaureate training in microbiology research and opportunities for professional development, which will prepare the candidate for graduate studies in microbiology.