Development and Application of New Ionization Methods for Biological Mass Spectrometry

项目摘要 质谱是一种非常强大的生物分析技术， 对人类健康的分子理解产生了深远的影响， 疾病质量分析仪技术、解离技术、 激光和电离方法在很大程度上归因于质量的核心作用， 光谱分析在系统生物学领域发挥着重要作用。虽然质谱分析法 在过去的世纪里，它已经发展成为一种非常有效的分析工具， 创新的重大机遇，使一个更多样化的 生物学问题有待解决。该提案的核心是 开发用于生物质谱的新电离方法， 能够对几类生物分子进行组织成像。短- 这项建议的长期目标是进一步发展和从根本上 用真实的生物系统来理解这种创新的电离方法。 这些结果将为生物应用提供坚实的基础 将直接受益。在这种心态下，我们将开发和应用这些新的 电离方法在模型生物体中的组织成像，以获得 深入了解：1）缺血性卒中; 2）伤口愈合; 3）心脏代谢 疾病长期目标是建立这些新的电离方法， 一种使生物分析技术能够有效地解决人类 健康和疾病。 对拟议研究的公开说明 质谱（MS），与“分子称重”相关的科学， 对人类健康和疾病的研究产生了深远的影响， 癌症、心脏病、神经退行性疾病、神经发育，以及 自身免疫性疾病MS的先决条件是将中性分子转化为 带电物质（离子），以便它们可以通过质量“称重” 光谱仪，并通过先进的分析技术鉴定。的重点 研究是开发新的电离方法，允许更多样化的阵列 当代生物医学问题有待解决。这将包括 组织成像，以最终提供对中风、创伤 愈合和心脏代谢疾病。

PROJECT SUMMARY Mass spectrometry is an extraordinarily powerful bioanalytical technique that has had a profound impact on our molecular understanding of human health and disease. Major advances in mass analyzer technology, dissociation techniques, lasers, and ionization methods are largely attributed to the central role that mass spectrometry plays in the field of systems biology. While mass spectrometry has evolved over the last century into a highly effective analytical tool, there remain significant opportunities for innovation, allowing an even more diverse array of biological questions to be addressed. This proposal is centered on the development of new ionization methods for biological mass spectrometry to enable tissue imaging across several classes of biological molecules. The short- term objective of this proposal is to further develop and fundamentally understand this innovative ionization method using real biological systems. These results will provide a solid foundation from which biological applications will directly benefit. In this mindset, we will develop and apply these new ionization methods to tissue imaging in model organisms to gain mechanistic insights into, 1) ischemic stroke; 2) wound healing; and 3) cardiometabolic disease. The long-term objective is to establish these new ionization methods as an enabling bioanalytical technology to effectively address questions in human health and disease. Public Description of Proposed Research Mass spectrometry (MS), the science related to the “weighing of molecules”, has had a profound impact on the study of human health and disease including cancer, heart disease, neurodegenerative diseases, neural development, and auto-immune diseases. A prerequisite of MS is to convert neutral molecules into charged species (ions) such that they can be “weighed” by the mass spectrometer and identified by advanced analytical techniques. The focus of this research is to develop new ionization methods allowing a more diverse array of contemporary biomedical questions to be addressed. This will include the imaging of tissues to ultimately provide new biological insights into stroke, wound healing and cardiometabolic disease.