Development and Application of New Ionization Methods for Biological Mass Spectrometry
生物质谱新型电离方法的开发与应用
基本信息
- 批准号:10349766
- 负责人:
- 金额:$ 54.67万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2009
- 资助国家:美国
- 起止时间:2009-07-01 至 2026-03-31
- 项目状态:未结题
- 来源:
- 关键词:3-DimensionalAblationAddressAirAlgorithmsAnimal ModelAtmospheric PressureAutoimmune DiseasesBiologicalCalibrationCardiometabolic DiseaseChargeChemicalsCodeCollaborationsCommunitiesComputer softwareCryoultramicrotomyDataData AnalysesData SetDatabasesDevelopmentDiseaseDissociationEducational workshopElectrospray IonizationEngineeringEnsureEvolutionFiber OpticsFoundationsFundingGoalsHealthHeart DiseasesHumanImageImaging technologyInformaticsIschemic StrokeLasersLicensingLightLinkLipidsMalignant NeoplasmsMass Spectrum AnalysisMeasurementMicroscopyMolecularMotionMultimodal ImagingNeurodegenerative DisordersNeurotransmittersOpticsPaperPathway interactionsPeptidesPhysiologicalPlayPolysaccharidesPositioning AttributePreparationProcessPublishingRecording of previous eventsResearchResolutionResource SharingRoleSamplingScienceSeriesShapesSkin wound healingSolidSolventsSourceSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSpottingsStrokeSystems BiologyTechniquesTechnologyThree-Dimensional ImagingTimeTissue imagingTissuesTranslatingVariantVendorZebrafishadvanced analyticsanalytical toolbasebiological systemsbrain tissuedetection limitexperienceimaging modalityimaging platformimprovedimproved functioninginnovationinsightionization techniquemachine learning algorithmmass analyzermass spectrometermass spectrometric imagingmultidimensional dataneurodevelopmentnew technologynext generationnovelopen sourceoperationpreservationsingle cell analysissoftware developmentwhole body imagingwound healing
项目摘要
PROJECT SUMMARY
Mass spectrometry is an extraordinarily powerful bioanalytical technique that has
had a profound impact on our molecular understanding of human health and
disease. Major advances in mass analyzer technology, dissociation techniques,
lasers, and ionization methods are largely attributed to the central role that mass
spectrometry plays in the field of systems biology. While mass spectrometry has
evolved over the last century into a highly effective analytical tool, there remain
significant opportunities for innovation, allowing an even more diverse array of
biological questions to be addressed. This proposal is centered on the
development of new ionization methods for biological mass spectrometry to
enable tissue imaging across several classes of biological molecules. The short-
term objective of this proposal is to further develop and fundamentally
understand this innovative ionization method using real biological systems.
These results will provide a solid foundation from which biological applications
will directly benefit. In this mindset, we will develop and apply these new
ionization methods to tissue imaging in model organisms to gain mechanistic
insights into, 1) ischemic stroke; 2) wound healing; and 3) cardiometabolic
disease. The long-term objective is to establish these new ionization methods as
an enabling bioanalytical technology to effectively address questions in human
health and disease.
Public Description of Proposed Research
Mass spectrometry (MS), the science related to the “weighing of molecules”, has
had a profound impact on the study of human health and disease including
cancer, heart disease, neurodegenerative diseases, neural development, and
auto-immune diseases. A prerequisite of MS is to convert neutral molecules into
charged species (ions) such that they can be “weighed” by the mass
spectrometer and identified by advanced analytical techniques. The focus of this
research is to develop new ionization methods allowing a more diverse array of
contemporary biomedical questions to be addressed. This will include the
imaging of tissues to ultimately provide new biological insights into stroke, wound
healing and cardiometabolic disease.
项目摘要
质谱法是一种非常强大的生物分析技术,
对人类健康的分子理解产生了深远的影响,
疾病质量分析仪技术、解离技术、
激光和电离方法在很大程度上归因于质量的核心作用,
光谱分析在系统生物学领域发挥着重要作用。虽然质谱分析法
在过去的世纪里,它已经发展成为一种非常有效的分析工具,
创新的重大机遇,使一个更多样化的
生物学问题有待解决。该提案的核心是
开发用于生物质谱的新电离方法,
能够对几类生物分子进行组织成像。短-
这项建议的长期目标是进一步发展和从根本上
用真实的生物系统来理解这种创新的电离方法。
这些结果将为生物应用提供坚实的基础
将直接受益。在这种心态下,我们将开发和应用这些新的
电离方法在模型生物体中的组织成像,以获得
深入了解:1)缺血性卒中; 2)伤口愈合; 3)心脏代谢
疾病长期目标是建立这些新的电离方法,
一种使生物分析技术能够有效地解决人类
健康和疾病。
对拟议研究的公开说明
质谱(MS),与“分子称重”相关的科学,
对人类健康和疾病的研究产生了深远的影响,
癌症、心脏病、神经退行性疾病、神经发育和
自身免疫性疾病MS的先决条件是将中性分子转化为
带电物质(离子),以便它们可以通过质量“称重”
光谱仪,并通过先进的分析技术鉴定。的重点
研究是开发新的电离方法,允许更多样化的阵列
当代生物医学问题有待解决。这将包括
组织成像,以最终提供对中风、创伤
愈合和心脏代谢疾病。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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David C. Muddiman其他文献
In celebration of the 70th birthday of Dr David M. Hercules
- DOI:
10.1007/s00216-002-1385-9 - 发表时间:
2002-07-06 - 期刊:
- 影响因子:3.800
- 作者:
Joseph A. Gardella;Fred E. Lytle;David C. Muddiman - 通讯作者:
David C. Muddiman
In-depth characterization of <em>N</em>-glycosylation and sialic acid content in fetal and adult fibrinogen
- DOI:
10.1016/j.rpth.2024.102618 - 发表时间:
2024-11-01 - 期刊:
- 影响因子:
- 作者:
Tana V. Palomino;Anastasia Sheridan;David C. Muddiman;Ashley C. Brown - 通讯作者:
Ashley C. Brown
High-resolution mass spectrometry
- DOI:
10.1007/s00216-012-5959-x - 发表时间:
2012-04-10 - 期刊:
- 影响因子:3.800
- 作者:
Hans H. Maurer;David C. Muddiman - 通讯作者:
David C. Muddiman
Three-dimensional mass spectrometry imaging (3D MSI): incorporating top-hat IR-MALDESI and automatic z-axis correction
- DOI:
10.1007/s00216-025-05755-w - 发表时间:
2025-02-03 - 期刊:
- 影响因子:3.800
- 作者:
Alexandria L. Sohn;John G. Witherspoon;Robert C. Smart;David C. Muddiman - 通讯作者:
David C. Muddiman
Topographic imaging with automatic z-axis correction of Brassica oleracea var. viridis leaves by IR-MALDESI mass spectrometry imaging
- DOI:
10.1007/s00216-025-05820-4 - 发表时间:
2025-03-15 - 期刊:
- 影响因子:3.800
- 作者:
Quinn Mills;Sarah M. Ashbacher;Alexandria L. Sohn;David C. Muddiman - 通讯作者:
David C. Muddiman
David C. Muddiman的其他文献
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{{ truncateString('David C. Muddiman', 18)}}的其他基金
Core of Advanced Platform Technologies Used for Remediation and Exploration
用于修复和勘探的先进平台技术核心
- 批准号:
10337306 - 财政年份:2020
- 资助金额:
$ 54.67万 - 项目类别:
Analysis of the cellular secretome by targeted subcellular proteomics
通过靶向亚细胞蛋白质组学分析细胞分泌组
- 批准号:
8884569 - 财政年份:2014
- 资助金额:
$ 54.67万 - 项目类别:
Analysis of the cellular secretome by targeted subcellular proteomics
通过靶向亚细胞蛋白质组学分析细胞分泌组
- 批准号:
8770157 - 财政年份:2014
- 资助金额:
$ 54.67万 - 项目类别:
Development and Application of Novel Glycan-Specific Reagents to Facilitate Early
新型聚糖特异性试剂的开发和应用以促进早期诊断
- 批准号:
8542502 - 财政年份:2011
- 资助金额:
$ 54.67万 - 项目类别:
Development and Application of Novel Glycan-Specific Reagents to Facilitate Early
新型聚糖特异性试剂的开发和应用以促进早期诊断
- 批准号:
8728430 - 财政年份:2011
- 资助金额:
$ 54.67万 - 项目类别:
2011 US-HUPO Conference -- Proteomics: New Developments and Grand Challenges
2011年US-HUPO会议——蛋白质组学:新进展与重大挑战
- 批准号:
8128049 - 财政年份:2011
- 资助金额:
$ 54.67万 - 项目类别:
Development and Application of New Ionization Methods for Biological Mass Spectrometry
生物质谱新型电离方法的开发与应用
- 批准号:
9252471 - 财政年份:2010
- 资助金额:
$ 54.67万 - 项目类别:
Development and Application of New Ionization Methods for Biological Mass Spectrometry
生物质谱新型电离方法的开发与应用
- 批准号:
10598032 - 财政年份:2009
- 资助金额:
$ 54.67万 - 项目类别:
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