Development and Application of New Ionization Methods for Biological Mass Spectrometry

生物质谱新型电离方法的开发与应用

• 批准号: 10349766
• 负责人: David C. Muddiman
• 金额: $ 54.67万
• 依托单位: NORTH CAROLINA STATE UNIVERSITY RALEIGH
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2026-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10349766
• 关键词: 3-Dimensional; Ablation; Address; Air; Algorithms; Animal Model; Atmospheric Pressure; Autoimmune Diseases; Biological; Calibration; Cardiometabolic Disease; Charge; Chemicals; Code; Collaborations; Communities; Computer software; Cryoultramicrotomy; Data; Data Analyses; Data Set; Databases; Development; Disease; Dissociation; Educational workshop; Electrospray Ionization; Engineering; Ensure; Evolution; Fiber Optics; Foundations; Funding; Goals; Health; Heart Diseases; Human; Image; Imaging technology; Informatics; Ischemic Stroke; Lasers; Licensing; Light; Link; Lipids; Malignant Neoplasms; Mass Spectrum Analysis; Measurement; Microscopy; Molecular; Motion; Multimodal Imaging; Neurodegenerative Disorders; Neurotransmitters; Optics; Paper; Pathway interactions; Peptides; Physiological; Play; Polysaccharides; Positioning Attribute; Preparation; Process; Publishing; Recording of previous events; Research; Resolution; Resource Sharing; Role; Sampling; Science; Series; Shapes; Skin wound healing; Solid; Solvents; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spottings; Stroke; Systems Biology; Techniques; Technology; Three-Dimensional Imaging; Time; Tissue imaging; Tissues; Translating; Variant; Vendor; Zebrafish; advanced analytics; analytical tool; base; biological systems; brain tissue; detection limit; experience; imaging modality; imaging platform; improved; improved functioning; innovation; insight; ionization technique; machine learning algorithm; mass analyzer; mass spectrometer; mass spectrometric imaging; multidimensional data; neurodevelopment; new technology; next generation; novel; open source; operation; preservation; single cell analysis; software development; whole body imaging; wound healing

PROJECT SUMMARY Mass spectrometry is an extraordinarily powerful bioanalytical technique that has had a profound impact on our molecular understanding of human health and disease. Major advances in mass analyzer technology, dissociation techniques, lasers, and ionization methods are largely attributed to the central role that mass spectrometry plays in the field of systems biology. While mass spectrometry has evolved over the last century into a highly effective analytical tool, there remain significant opportunities for innovation, allowing an even more diverse array of biological questions to be addressed. This proposal is centered on the development of new ionization methods for biological mass spectrometry to enable tissue imaging across several classes of biological molecules. The short- term objective of this proposal is to further develop and fundamentally understand this innovative ionization method using real biological systems. These results will provide a solid foundation from which biological applications will directly benefit. In this mindset, we will develop and apply these new ionization methods to tissue imaging in model organisms to gain mechanistic insights into, 1) ischemic stroke; 2) wound healing; and 3) cardiometabolic disease. The long-term objective is to establish these new ionization methods as an enabling bioanalytical technology to effectively address questions in human health and disease. Public Description of Proposed Research Mass spectrometry (MS), the science related to the “weighing of molecules”, has had a profound impact on the study of human health and disease including cancer, heart disease, neurodegenerative diseases, neural development, and auto-immune diseases. A prerequisite of MS is to convert neutral molecules into charged species (ions) such that they can be “weighed” by the mass spectrometer and identified by advanced analytical techniques. The focus of this research is to develop new ionization methods allowing a more diverse array of contemporary biomedical questions to be addressed. This will include the imaging of tissues to ultimately provide new biological insights into stroke, wound healing and cardiometabolic disease.

项目摘要 质谱是一种非常强大的生物分析技术，具有 对我们对人类健康的分子理解产生了深远的影响 疾病。大众分析仪技术，解离技术的重大进展， 激光和电离方法在很大程度上归因于质量的中心作用 光谱法在系统生物学领域发挥作用。而质谱有 上个世纪发展成为一种高效的分析工具，仍然存在 创新的巨大机会，允许更多的潜水员阵列 生物学问题要解决。该建议集中在 开发用于生物质谱的新电离方法 在几类生物分子中启用组织成像。短 - 该提案的术语目标是进一步发展和从根本上发展 使用实际生物系统了解这种创新的电离方法。 这些结果将为生物应用提供坚实的基础 将直接受益。在这种心态中，我们将开发并应用这些新的 模型生物中组织成像的电离方法以获取机械 深入了解，1）缺血性中风； 2）伤口愈合； 3）心脏代谢 疾病。长期目标是将这些新的电离方法建立为 一种使生物分析技术有效解决人类问题的问题 健康与疾病。 拟议研究的公开描述 质谱法（MS），与“分子称重”有关的科学具有 对人类健康和疾病的研究产生了深远影响 癌症，心脏病，神经退行性疾病，神经退行性疾病，神经发育和 自动免疫疾病。 MS的先决条件是将中性分子转换为 带电物种（离子），使它们可以被质量“称重” 光谱仪并通过高级分析技术识别。重点 研究是开发新的电离方法，允许更多多样化 当代生物医学问题要解决。这将包括 组织成像，最终为中风，伤口提供新的生物学见解 愈合和心脏代谢疾病。