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Development and Application of New Ionization Methods for Biological Mass Spectrometry

生物质谱新型电离方法的开发与应用

基本信息

批准号：
10349766
负责人：
David C. Muddiman
金额：
$ 54.67万
依托单位：
NORTH CAROLINA STATE UNIVERSITY RALEIGH
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-07-01 至 2026-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10349766
关键词：
3-Dimensional Ablation Address Air Algorithms Animal Model Atmospheric Pressure Autoimmune Diseases Biological Calibration Cardiometabolic Disease Charge Chemicals Code Collaborations Communities Computer software Cryoultramicrotomy Data Data Analyses Data Set Databases Development Disease Dissociation Educational workshop Electrospray Ionization Engineering Ensure Evolution Fiber Optics Foundations Funding Goals Health Heart Diseases Human Image Imaging technology Informatics Ischemic Stroke Lasers Licensing Light Link Lipids Malignant Neoplasms Mass Spectrum Analysis Measurement Microscopy Molecular Motion Multimodal Imaging Neurodegenerative Disorders Neurotransmitters Optics Paper Pathway interactions Peptides Physiological Play Polysaccharides Positioning Attribute Preparation Process Publishing Recording of previous events Research Resolution Resource Sharing Role Sampling Science Series Shapes Skin wound healing Solid Solvents Source Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spottings Stroke Systems Biology Techniques Technology Three-Dimensional Imaging Time Tissue imaging Tissues Translating Variant Vendor Zebrafish advanced analytics analytical tool base biological systems brain tissue detection limit experience imaging modality imaging platform improved improved functioning innovation insight ionization technique machine learning algorithm mass analyzer mass spectrometer mass spectrometric imaging multidimensional data neurodevelopment new technology next generation novel open source operation preservation single cell analysis software development whole body imaging wound healing

项目摘要

PROJECT SUMMARY Mass spectrometry is an extraordinarily powerful bioanalytical technique that has had a profound impact on our molecular understanding of human health and disease. Major advances in mass analyzer technology, dissociation techniques, lasers, and ionization methods are largely attributed to the central role that mass spectrometry plays in the field of systems biology. While mass spectrometry has evolved over the last century into a highly effective analytical tool, there remain significant opportunities for innovation, allowing an even more diverse array of biological questions to be addressed. This proposal is centered on the development of new ionization methods for biological mass spectrometry to enable tissue imaging across several classes of biological molecules. The short- term objective of this proposal is to further develop and fundamentally understand this innovative ionization method using real biological systems. These results will provide a solid foundation from which biological applications will directly benefit. In this mindset, we will develop and apply these new ionization methods to tissue imaging in model organisms to gain mechanistic insights into, 1) ischemic stroke; 2) wound healing; and 3) cardiometabolic disease. The long-term objective is to establish these new ionization methods as an enabling bioanalytical technology to effectively address questions in human health and disease. Public Description of Proposed Research Mass spectrometry (MS), the science related to the “weighing of molecules”, has had a profound impact on the study of human health and disease including cancer, heart disease, neurodegenerative diseases, neural development, and auto-immune diseases. A prerequisite of MS is to convert neutral molecules into charged species (ions) such that they can be “weighed” by the mass spectrometer and identified by advanced analytical techniques. The focus of this research is to develop new ionization methods allowing a more diverse array of contemporary biomedical questions to be addressed. This will include the imaging of tissues to ultimately provide new biological insights into stroke, wound healing and cardiometabolic disease.

项目总结