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Development and Application of New Ionization Methods for Biological Mass Spectrometry

生物质谱新型电离方法的开发与应用

基本信息

批准号：
9252471
负责人：
David C. Muddiman
金额：
$ 31.82万
依托单位：
NORTH CAROLINA STATE UNIVERSITY RALEIGH
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-09-01 至 2021-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9252471
关键词：
Ablation Address Algorithms Antineoplastic Agents Atmospheric Pressure Autoimmune Diseases Biological Biological Models Biology Cancer Model Cell surface Charge Chemicals Computer software Custom Data Databases Defect Detection Development Disease Dissociation Drug Kinetics Electrospray Ionization Evolution Foundations Funding Genetically Engineered Mouse Health Heart Diseases Human Image Image Analysis Imaging technology Informatics Ions Knockout Mice Lasers Licensing Lipids Liquid substance Malignant Neoplasms Malignant neoplasm of ovary Mass Spectrum Analysis Mediating Methodology Microdissection Microscopy Modality Molecular Multimodal Imaging Ovary Pathway interactions Pattern Penetration Performance Pharmaceutical Preparations Physiological Play Polysaccharides Preparation Problem Solving Proteins Research Resolution Role Sampling Science Shotguns Solid Source Specimen Structure Systems Biology Techniques Technology Three-Dimensional Imaging Tissue imaging Tissues Tumor Tissue Variant Vendor analytical tool biological systems design image processing imaging modality imaging platform imaging study improved innovation insight instrumentation ionization ionization technique mass analyzer mass spectrometer mouse model neurodevelopment neuron development novel open source operation optical imaging public health relevance reconstruction software development tool

项目摘要

DESCRIPTION (provided by applicant): Mass spectrometry is an extraordinarily powerful bioanalytical technique that has had a profound impact on our molecular understanding of human health and disease. Major advances in mass analyzer technology, dissociation techniques, and ionization methods are largely attributed to the central role that mass spectrometry plays in the field of systems biology. While mass spectrometry has evolved over the last century into a highly effective analytical tool, there are still opportunities for new advances to be made allowing an even more diverse array of biological questions to be addressed. This proposal is centered on the development of new ionization methods for biological mass spectrometry to allow for tissue imaging across several classes of biological molecules. The short-term objective of this proposal is to further develop and fundamentally understand this innovative ionization method using real biological systems. These results will provide a solid foundation from which biological applications will directly benefit. In this mindse, we will develop and apply these new ionization methods to tissue imaging. The long-term objective is to establish these new ionization methods as an enabling bioanalytical technology to effectively address questions in human health and disease. Mass spectrometry (MS), the science related to the "weighing of molecules", has had a profound impact on the study of human health and disease including cancer, heart disease, neural development, and auto-immune diseases. A prerequisite of MS is to convert neutral molecules into charged species (ions) such that they can be "weighed" by the mass spectrometer. The focus of this research is to develop new ionization methods allowing a more diverse array of contemporary biomedical questions to be addressed. This will include the imaging of tissues to ultimately provide new biological insights.