Establish and Characterize an Air Liquid Interface In Vitro Exposure System

转化毒理学部门（DTT）继续优化使用政府拥有的Vitrocell 48 2.0 plus暴露系统，为该项目提供模拟吸入暴露的体外能力。暴露的吸入途径在空气污染物和工业环境中很常见，直到最近还很难在体外模拟。该系统允许对吸入暴露的潜在有毒物质进行体外测试，这有助于减少动物使用的计划目标。该系统提供高通量，可用于空气中的物质，如蒸汽，液体气溶胶，干粉和纤维的复杂混合物。数字地面电视正在对该系统进行进一步的评估，以增加对该系统将按预期运作的信心。这将通过设计和实施系统表征以及对选定的试验剂进行试点体外毒性测试来实现，这些测试剂具有可靠的体内相关性数据，使用来自代表性人群的组织。该系统还允许对人类和老鼠的细胞进行测试，以提供支持物种外推的数据。

The Division of Translational Toxicology (DTT) continued to optimize use of the government owned Vitrocell 48 2.0 plus exposure system to provide the program with in vitro capabilities that mimic inhalation exposure. The inhalation route of exposure is commonly seen among air pollutants as well as with industrial settings, and until recently has been very difficult to simulate in vitro. This system allows in vitro testing of potential toxicants for inhalation exposure which contributes to the program's goals of reducing the use of animals. This system provides high throughput and can be used for airborne substances such as vapors, complex mixtures of liquid aerosol, dry powders, and fibers. DTT is conducting additional evaluation of the system to increase confidence that the system will function as intended. This will be achieved through the design and conduct of system characterization and pilot in vitro toxicity testing of selected test agents with robust in vivo data for correlation, using tissue from representative human populations. The system also allows testing of cells from humans and rats to provide data supporting species extrapolation. Keyword: in vitro, inhalation exposure