Hybrid Rat Diversity Program - cryopreservation resource for rat models

项目摘要 这个项目的持续目标是将大鼠模型用于临床转化 通过建立动物资源和基因组数据集， 实验室研究与临床相关性。杂交大鼠多样性小组（HRDP） 资源为研究社区提供了系统遗传学的基础和工具， 遗传学前沿HRDP亲本奖旨在1）继续建立96菌株 杂交大鼠多样性小组包括33个经典近交系，30个HXB/BXH重组体 胚再衍生的33个FXLE/LEXF重组自交系， 进口，2）表征，维护和分发HRDP，3）提供基因组和 HRDP的转录组测序和4），并提供数据分析，整合和 主要通过大鼠基因组数据库（RGD）进行传播。具体来说，这一补充 将1）评估冷冻保存前后的胚胎质量，2）提高 收集胚胎进行冷冻保存，以及3）开发定制的冷冻保存方案， 菌株特性这些结果将对科学界产生广泛的效用 通过选择的菌株中的遗传和表型多样性， 补充项目结合HRDP母公司奖励的可用数据。数据 和协议将提供给研究界通过大鼠基因组 数据库，出版物，和通过鼠库。

Project Summary The ongoing goal of this program is to extend the use of rat models for clinical translation through establishing an animal resource and genomic dataset to facilitate the translation of laboratory research to clinical relevance. Specifically, the Hybrid Rat Diversity Panel (HRDP) resource provides the research community with a foundation for systems genetics and a tool for forward genetics. The HRDP parent award aims to 1) to continue to establish the 96 strain Hybrid Rat Diversity Panel comprised of 33 classic inbred strains, 30 HXB/BXH recombinant inbred strains and 33 FXLE/LEXF recombinant inbred strains through embryo rederivation and importation, 2) to characterize, maintain and distribute the HRDP, 3) to provide genomic and transcriptomic sequencing of the HRDP and 4) and provide data analysis, integration and dissemination primarily through the Rat Genome Database (RGD). Specifically, this supplement will 1) evaluate embryo quality pre- and post-cryopreservation, 2) improve the efficiency of embryo collection for cryopreservation, and 3) develop cryopreservation protocols customized to strain characteristics. Together these results will have broad utility the scientific community through the genetic and phenotypic diversity represented in the strains selected for the supplement project in combination with the available data from the HRDP parent award. Data and protocols will be made available to the research community through the Rat Genome Database, publications, and through rat repositories.