Flow Cytometry Shared Resource

流式细胞仪共享资源（FCSR） 项目总结/摘要 流式细胞术共享资源（FCSR）提供最先进的细胞分选和分析细胞术， 以及细胞计数教育，加州大学戴维斯分校综合癌症中心（UCDCCC）社区。的 FCSR是UCD唯一开放的流式细胞术核心实验室，由细胞术专家组成，他们 随时可用，以帮助用户进行实验规划、仪器培训、操作员辅助单元 分类、数据分析、故障排除、手稿准备和审查，以及高级数据分析，使用 流行的FlowJo流式细胞仪分析软件中先进的主成分分析工具 包. FCSR在三个地点设有设施，一个在萨克拉门托的加州大学戴维斯分校健康校园，两个在 戴维斯校园，提供各种各样的细胞仪设备。萨克拉门托设施设有BD Fortessa、BD FACS Canto I和ESDIGM S1000 EX分析型细胞仪，以及BD inFlux和Aria II细胞 分拣员戴维斯工厂拥有一台新的Cytek Aurora光谱细胞分析仪，Becton Dickinson LSR-II， FACScan和Beckman Coulter Cytoflex细胞分析仪以及Astrios和6色BD FACSCanto II细胞分选仪， 和用于单细胞中基因表达分析的Fluidigm BioMark系统。FCSR支持广泛的 在UCDCCC的基础研究项目，旨在阐明癌症病因学，转移， 进展和治疗。BRSR的监督和管理由主任Barbara L. Shacklett博士是一位免疫学家和病毒学家，拥有20多年的流式细胞术经验。 方法在她的研究;和技术总监，布里奇特麦克劳克林，MS，一个公认的校园领导者 在细胞计数技术、仪器和教育方面拥有20年的经验， 流式细胞术和分析。社会服务中心的工作人员定期与UCDCCC成员协商， 开发细胞分析和分选的新方法。该中心提供年度综合课程， 在流式细胞术，包括流式细胞术的基础知识，先进的主题，并动手培训。的 UCDCCC领导赞助奖学金，为学生进行癌症相关的研究。具体 FCSR的目标是：1）提供最先进的细胞分选和分析细胞术。2)建议用户 适当的实验设计和数据分析。3)教育用户关于常规以及切割- 边缘细胞计数应用。4)利用这些技术促进研究人员之间的互动。5)刺激 发展癌症研究的新方法。

FLOW CYTOMETRY SHARED RESOURCE (FCSR) PROJECT SUMMARY/ABSTRACT The Flow Cytometry Shared Resource (FCSR) provides state-of-the-art cell sorting and analytical cytometry, as well as cytometry education, to the UC Davis Comprehensive Cancer Center (UCDCCC) community. The FCSR is the only openly available flow cytometry core laboratory at UCD staffed by cytometry experts who are readily available to assist users with experiment planning, instrumentation training, operator-assisted cell sorting, data analysis, troubleshooting, manuscript preparation and review, and advanced data analysis, using the advanced principal component analysis tools in the popular FlowJo flow cytometry analysis software package. FCSR has facilities at three sites, one at the UC Davis Health campus in Sacramento, and two on the Davis campus, offering a wide variety of cytometry equipment. The Sacramento facility houses the BD Fortessa, BD FACS Canto I, and Stratedigm S1000EX analytic cytometers, and the BD inFlux and Aria II cell sorters. The Davis facilities house a new Cytek Aurora spectral cell analyzer, Becton Dickinson LSR-II, FACScan, and Beckman Coulter Cytoflex cell analyzers, and Astrios and 6-color BD FACSCanto II cell sorters, and a Fluidigm BioMark system for gene expression analysis in single cells. The FCSR supports a wide range of basic research projects at UCDCCC aimed at elucidating mechanisms in cancer etiology, metastasis, progression, and treatment. Oversight and management of the BRSR is provided by the Director, Barbara L. Shacklett, PhD an immunologist and virologist with over 20 years of experience using flow cytometry approaches in her research; and the technical director, Bridget McLaughlin, MS, a recognized campus leader in with 20 years of experience in cytometry techniques, instrumentation and education and particular expertise in multicolor flow cytometry and analysis. FCSR personnel regularly consult with UCDCCC members to help develop new approaches for cellular analysis and sorting. The FCSR offers an annual comprehensive course in Flow Cytometry that includes flow cytometry fundamentals, advanced topics, and hands on training. The UCDCCC leadership sponsors scholarships for students conducting cancer-related research. The Specific Aims for the FCSR are to: 1) Provide state-of-the-art cell sorting and analytical cytometry. 2) Advise users about appropriate experimental design and data analysis. 3) Educate users about routine as well as cutting- edge cytometry applications. 4) Facilitate interactions among researchers using these techniques. 5) Stimulate development of new approaches for cancer research.