Administrative supplement to Duke G20 award

项目摘要/摘要： 杜克地区生物遏制实验室(RBL)是由国家研究所提供资金建造的 卫生部(NIH)支持必要的基础研究，以开发药物、诊断和疫苗 新出现/再次出现的感染和生物防御。最先进的生物遏制设施，旨在 支持实验室和小动物研究，于2007年在杜克完全委托并开放运营 北卡罗来纳州达勒姆的大学医学院校区。杜克大学RBL基金支持赠款/合同资助 研究人员还提供生物遏制实验室、创新的宿主监测分析和小动物模型 在收回成本的基础上通过核心服务中心单位。十四年的使用和时间使 杜克大学RBL的一些设施、系统和关键核心研究设备陈旧或需要维修， 更替或现代化。自从母公司G20获奖以来，我们已经确定了更多关键的Defi 该设施现有的有形基础设施限制了生物安全、安保或威胁到其提供的服务 它支持的优惠和研究相关活动。我们将通过以下方式解决这些额外的缺陷 在我们最初的三个具体目标中补充资金：1)使建筑控制系统现代化--更换和 升级陈旧的空压机和BACnet(楼宇自动化控制网络)；2)现代化 生物安全和安保系统-更换陈旧/损坏的PAPR装置；以及3)使研究资源现代化 -更换或增加杜克RBL研究服务核心的其他主要设备-液体处理 自动化设备，ELISpot阅读器，下一代Luminex阅读器，升级细胞计数器，更换 孵化器、冷冻机和离心机，BSL3的高级荧光显微镜功能，额外的Allentown 具有微型CT功能的生物围栏、老鼠笼子和活体动物成像。这些目标/目标将是 由高度集成的项目管理团队在父奖项中与Solid合作完成 机构支持和值得信赖的承包商/供应商是原始设计和建造的一部分 设施。

Project Summary/Abstract: The Duke Regional Biocontainment Laboratory (RBL) was constructed with funding from the National Institutes of Health (NIH) to support basic research necessary to develop drugs, diagnostics, and vaccines for emerging/reemerging infections and biodefense. The state-of-the-art biocontainment facility, designed to support lab and small animal research, was fully commissioned and opened for operation in 2007 on the Duke University Medical School Campus in Durham, NC. The Duke RBL facility supports grant/contract-funded investigators and also provides biocontainment labs, innovative host monitoring assays and small animal models through Core Service Center Units on a cost recovery basis. Fourteen years of use and time have rendered some of the Duke RBL facilities, systems and critical core research equipment obsolete, or in need of repair, replacement or modernization. Since award of the parent G20 we have identified additional critical deficiencies of the facility’s existing physical infrastructure that limit biosafety, security or threaten the provision of services it offers and research-related activities it supports. We will address these additional deficiencies through this supplement funding in our three original Specific Aims: 1) Modernize Building Control Systems – Replace and upgrade obsolete air compressor and the BACNet (Building Automation Control Network); 2) Modernize Biosafety and Security Systems - Replace aged/damaged PAPR units; and 3) Modernize Research Resources – Replace or add additional major equipment to the Duke RBL Research Service Cores - liquid handling automation equipment, ELISpot reader, next-generation Luminex reader, upgraded cell counter, replacement incubators, freezers and centrifuges, advanced fluorescence microscopy capability in BSL3, additional Allentown Biocontainment mouse caging, and live animal imaging with micro CT capability. These Aims/Goals will be accomplished by the highly integrated project management team assembled in the parent award with solid institutional support and trusted contractors/vendors that were part of the original design and construction of the facility.