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Gene Transfer Core, NIDCR-DIR

Gene Transfer Core, NIDCR-DIR
基因转移核心，NIDCR-DIR
批准号：
10920222
负责人：
Ashok Kulkarni
金额：
$80.26万
依托单位：
NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/10920222
关键词：
Biological AssayBiopsyClone CellsClustered Regularly Interspaced Short Palindromic RepeatsConsultationsCryopreservationEmbryoEmbryo TransferGene TargetingGene TransferGenerationsGenesGenomic DNAGenotypeInjectionsKnock-in MouseKnock-outKnockout MiceMusNational Institute of Dental and Craniofacial ResearchPrincipal InvestigatorResearchResearch PersonnelServicesTailTransgenic MiceTransgenic Organismsembryonic stem cellexperimental studygain of functionloss of functionmouse modelpreservationsperm cellsperm cryopreservationtool

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中文摘要
翻译
在2013财年，基因转移核心成功地提供了以下服务： 原核注射服务：注射7个基于CRISPR的基因编辑构建体，产生34个转基因株系。 基于CRISPR的基因编辑服务：基于CRISPR的构建物生成KO和KI小鼠的平均效率分别为25%和19%。 冷冻保存和体外受精服务：对36只小鼠模型的精子进行冷冻保存，供9名首席研究人员(21名研究人员)使用。所有小鼠模型的精子都被储存在两个独立的建筑中。16根精子管解冻52次体外受精。 基因分型服务：对587例尾部活检标本进行纯化，用聚合酶链式反应进行基因分型。
英文摘要
In FY23, the Gene Transfer Core successfully provided following services: Pronuclear injection service: 7 CRISPR-based gene editing constructs were injected to generate 34 transgenic lines. CRISPR-based gene editing service: The average efficiency for generating KO and KI mice for CRISPR-based constructs was 25% and 19%, respectively. Cryopreservation and IVF services: Sperm from 36 mouse models were cryopreserved for 9 Principal investigators (21 investigators). Sperm from all mouse models were stored in two separate buildings. 16 sperm straws were thawed for 52 IVF sessions. Genotyping service: 587 tail biopsies were purified and used for genotyping by PCR analysis.
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