Einstein Mount Sinai Diabetes Research Center

稳定同位素和代谢组学核心（SIMC）的目标是向爱因斯坦山提供 西奈糖尿病研究中心的研究人员具有成本效益，高效和强大 用于测定代谢物/脂质的平台，旨在涵盖联锁代谢物 和脂质途径，并有助于提高我们对改变燃料的条件的理解 在胞质和线粒体代谢途径中的利用和生物合成，即，糖酵解， 戊糖和三羧酸（TCA）循环。为了在一个设施中实现这些目标，SIMC 更新了其技术基础设施，包括最新的海马XFe 24和XFe 96 海马通量分析仪用于真实的实时评估糖酵解和线粒体能量学， 最新的Sciex 6500 + QTRAP，具有硒离子迁移率，用于靶向LC/MS。MS代谢物和 脂质组学分析，并将其Orbitrap/LTQ高质量精度仪器更换为 最新的Shimadzu LC-9030四极杆飞行时间与Nexera UPLC用于非靶向 代谢组学和脂质组学分析。这些最新的增加增加了高质量分辨率 沃茨GC-TOF/MS和两个单位道尔顿分辨率Agilent单四极杆GC/MS机器 实现以下目标： 1.确定代谢组学/脂质组学特征，用于诊断/表征 细胞、组织和生物流体（血浆，尿， 凳子等）。 2.为了进行体内稳定同位素底物测定以确定蛋白质合成速率， 脂肪生成，外周葡萄糖处理，肝脏葡萄糖再循环，葡萄糖-甘油循环 和科里循环，高能磷酸盐（ATP，磷酸肌酸）周转，以及体外 代谢途径的稳定同位素通量剖析。 3.进行糖酵解评估（细胞外酸化率、糖酵解ATP 生产率）和线粒体耗氧量（线粒体呼吸和 线粒体ATP生产率）在组织外植体，原代分离和组织培养 使用Seahorse Biosciences Flux分析仪。 4.为ES-DRC研究人员及其学生、研究员和技术人员提供建议和指导 在设计和解释通量组学、代谢组学/脂质组学和计算 旨在阐明葡萄糖和脂质的分子基础的分析 在人类和动物模型中的稳态。

The goal of the Stable Isotope and Metabolomics Core (SIMC) is to provide to Einstein-Mount Sinai Diabetes Research Center research investigators cost effective, efficient and robust platforms for the determination of metabolites/lipids designed to cover interlocking metabolite and lipid pathways, and help to improve our understanding of conditions which change fuel utilization and biosynthesis in cytosolic and mitochondrial metabolic pathways, i.e., glycolysis, pentose and tricarboxylic acid (TCA) cycles. To accomplish these goals in one facility, the SIMC has updated its technology infrastructure to include the latest Seahorse XFe24 and XFe96 Seahorse Flux Analyzers for assessment of glycolytic and mitochondrial energetics in real time, the latest Sciex 6500+ QTRAP with Selexion ion mobility for targeted LC/MS.MS metabolite and lipidomic analyses, and is due to replace its Orbitrap/LTQ high mass accuracy instrument with the latest Shimadzu LC-9030 quadrupole Time of Flight with Nexera UPLC for untargeted metabolomic and lipidomic analyses. These latest additions add to the high mass resolution Waters GC-TOF/MS and two unit Dalton resolution Agilent single quadrupole GC/MS machines to accomplish the following aims: 1. To determine metabolomic/lipidomic profiles for the diagnosis/characterization of physiological and pathophysiological states in cells, tissues and biofluids (plasma, urine, stool, etc.). 2. To perform in vivo stable isotope substrate assays to determine rates of protein synthesis, lipogenesis, peripheral glucose disposal, hepatic glucose recycling, glucose-glycerol cycling and Cori cycling, high energy phosphate (ATP, creatine phosphate) turnover, and in vitro stable isotope flux dissections of metabolic pathways. 3. To perform assessments of glycolysis (extracellular acidification rates, glycolytic ATP production rates) and mitochondrial oxygen consumption (mitochondrial respiration and mitochondrial ATP production rates) in tissue explants, primary isolated and tissue culture cells using Seahorse Biosciences Flux Analyzers. 4. To advise and instruct ES-DRC investigators and their students, fellows and technical staff in the design and interpretation of fluxomics, metabolomics/lipidomics and computational analyses with the aim of elucidating the molecular basis underlying glucose and lipid homeostasis in humans and animal models.