Flow cytometry support to research in immune, skin, muscle and bone diseases

该设施目前支持许多NIAMS研究人员的研究。 特别是，它积极参与免疫调节组的研究项目，并直接参与了该组织暑期IRTA学生进行的研究项目，并提供培训和分析支持。 该设施容纳： 一台Beckman Coulter MoFlo高速分选仪（3激光激发，10色检测，细胞沉积单元）。 Beckman Coulter CyAn分析仪（3激光激发，9色检测） 一台BD Biosciences FACSCanto分析仪，带HTS（2激光激发，6色检测，高通量进样器）。 两台BD Biosciences FACSCalibur（x2）分析仪（2激光激发，4色检测） 新的BD Biosciences FACSAria II高速分选仪（3激光激发，12色检测，细胞沉积单元）也将很快上市。 迄今为止，已有超过35名NIAMS调查员接受了使用该设施现有不同平台的培训。 除了几个免疫学实验室的项目外，该设施还成功地将流式细胞术和分选服务扩展到NIAMS内的研究人员：Morasso博士，发育皮肤生物学单位，Wangs博士肌肉生物学实验室，特别是Tuans博士软骨生物学和骨科分支。这些项目在传统上不被认为适用于流式细胞术的领域，但最初相当令人鼓舞。 此外，Jim Simone，核心部门负责人，通过参与组织和促进季度会议，在NIH范围内参与促进流式细胞术，以展示该领域的领先研究人员，并促进与流式细胞术相关产品供应商的关系。

The facility currently supports the research of many NIAMS investigators. In particular, it is actively involved in the research projects of the Immunoregulation Group and has collaborated directly in the research project performed by a Summer IRTA student of the group, with training and assay support. The Facility houses: One Beckman Coulter MoFlo high-speed sorter (3-laser excitation, 10-color detection, cell deposition unit). One, Beckman Coulter CyAn analyzer (3-laser excitation, 9-color detection) One BD Biosciences FACSCanto analyzer w/HTS (2-laser excitation, 6-color detection, high-throughput sampler). Two BD Biosciences FACSCalibur (x2) analyzers (2-laser excitation, 4-color detection) A new BD Biosciences FACSAria II high-speed sorter (3-laser excitation, 12-color detection, cell deposition unit) will also soon be available. More than Thirty-five NIAMS investigators have been trained to date to use the different platforms available in the facility. Besides the projects from several immunology laboratories the facility has successfully expanded flow cytometry and sorting services to investigators within NIAMS: Dr. Morasso, Developmental Skin Biology Unit, , Dr. Wangs Laboratory of Muscle Biology and especially Dr. Tuans Cartilage Biology and Orthopaedics Branch. These projects are in areas not traditionally considered applicable to flow cytometry but have been initially quite encouraging. Furthermore, Jim Simone, the core Section Leader, has been involved in the promotion of flow cytometry at NIH-wide level by being involved in organizing and promoting quarterly meetings to showcase leading researchers in the field and promote relationships with vendors of flow cytometry-related products.