Parasite Genetics Core

摘要 寄生虫遗传核心(PGC)的目标将是使南部和中部非洲ICEMR能够 应用最先进的下一代测序和生物信息学工具更好地了解疟疾 赞比亚、津巴布韦和刚果民主共和国的流行病学和控制干预措施的影响 刚果(金)。具体地说，PGC的长期目标和目标是提供实验室分析 来自ICEMR样本的寄生虫，为以下项目的下一代测序数据进行生物信息处理 这一先前的目标是，对这一和其他ICEMR的调查人员进行培训，遗传数据的质量控制和 用于在实地进行分析的样本。PGC将提供一个集中的基因实验室 对ICEMR内所有三个项目的寄生虫样本进行分析。这些实验室分析将重点放在 主要介绍下一代测序技术：(1)扩增子深度测序，(2)混合扩增子深度测序 测序和(3)PoolSeq.PGC的拟议实验室工作包括以下活动：项目A： 南部和中部非洲的疟疾控制(流行病学)，项目B：难治疟疾病媒 南部和中部非洲的人口和残余传播(传播I)和项目C： 实现、维持和记录消灭疟疾(传播II)。NEXT的数据分析 世代排序是一种计算量大且发展迅速的方法。因此，核心的一部分将是 致力于协助调查人员进行这些最先进的分析。PGC的设施将允许我们 接待南部和中部非洲ICEMR的调查人员，并与调查人员合作 来自其他ICEMR的技术，以便将技术转移到其他机构。这将使我们能够 有可能促进跨ICEMR合作，并培训非美国科学家了解最先进的人口 遗传学实验室和分析方法。

Abstract The aim of the Parasite Genetics Core (PGC) will be to enable the Southern and Central Africa ICEMR to apply state-of-the-art next generation sequencing and bioinformatic tools to better understand malaria epidemiology and the impact of control interventions in Zambia, Zimbabwe and the Democratic Republic of the Congo (DRC). Specifically, the long term objectives and goals of the PGC are to provide; laboratory analyses of parasites from ICEMR samples, bioinformatic processing of next-generation sequencing data for projects in this previous aim, training for investigators in this and other ICEMRs, quality control of genetic data and samples for analyses conducted at field sites. The PGC will provide a centralized laboratory for genetic analysis of parasite samples from all three projects within the ICEMR. These laboratory analyses will focus primarily on next generation sequencing techniques: (1) amplicon deep sequencing, (2) pooled amplicon deep sequencing and (3) PoolSeq. The proposed laboratory work for the PGC includes activities in: Project A: Malaria Control in Southern and Central Africa (Epidemiology), Project B: Intractable Malaria Vector Populations and Residual Transmission in Southern and Central Africa (Transmission I), and Project C: Achieving, Sustaining, and Documenting Malaria Elimination (Transmission II). Data analysis of next generation sequencing is computationally intensive and rapidly developing. Thus, a portion of the core will be dedicated to assisting investigators with these state-of-the art analyses. The facilities at the PGC will allow us to host investigators from the Southern and Central Africa ICEMR, as well as collaborate with investigators from other ICEMRs, in order for the techniques to be transferred to other institutions. This will allow us to potentially foster cross ICEMR collaboration and to train non-US based scientists on state of the art population genetics laboratory and analysis methods.