Digital probabilistic MRI atlas of primate brain with toolkits for surgery guidan

描述(由申请人提供)：该项目的目标是开发一个全数字包，包括三维(3D)概率地图集、脑和头部坐标系以及用于侵入性程序指导和数据分析的工具包。将制作猕猴图集(Macaca Mulatta)。图谱一直在灵长类动物大脑的神经生物学研究中发挥着核心作用。与传统图谱相比，该图谱提供了(1)精确的活体解剖框架，(2)任意方位的2D切片，(3)便于数据映射的数字格式，(4)更具代表性的概率数据，(5)手术指导和数据分析工具包。将以皮质表面几何形状和白质纤维束成像为标准检查标记的准确性。这一数字资料包将免费分发，以方便对灵长类动物的神经生物学研究。将利用扩散张量磁共振显微成像技术建立高分辨率、高对比度的磁共振图谱。高对比度和高分辨率分别来源于扩散张量成像和显微成像。在数字地图集的基础上，将开发实验前手术指导工具包和实验后数据分析工具包，并将其纳入一个用户友好的单一平台。将通过这四个目标重点发展技术和数据库。首先，将获取高质量的体外和体内数据，并对体外数据进行登记。其次，大多数灰质和白质神经结构将被标记和分割。第三，通过将脑图像转换为模板来建立概率图谱和标记。第四，将开发一个用于指导侵入性操作和实验数据分析的平台。概率地图集是该提案的关键和核心部分。它以数字包的形式提供数据库。将建立大脑和头部坐标，以规范结构-位置关系体系。有了图集和坐标系，两个工具包为灵长类研究人员使用图集提供了方便和实用的手段。 与公共健康相关：拟议的灵长类三维数字概率地图集将提供关于灵长类大脑结构的准确、全面和不断演变的数字帐户。开发的工具包用于指导手术过程和绘制实验数据图，将为灵长类动物脑功能的研究带来精确、方便和高效的便利。灵长类动物大脑与人类大脑关系密切，对人类大脑功能的认识也将大大提高。

DESCRIPTION (provided by applicant): The goal of the project is to develop a fully digital package including three dimensional (3D) probabilistic atlas, brain and head coordinate systems and toolkits for invasive procedure guidance and data analysis. Atlas of macaque (Macaca mulatta) will be made. Atlases have been playing central roles for neurobiological studies on primate brains. Compared to conventional atlas, the proposed 3D digital probabilistic MRI atlas provides (1) accurate in vivo anatomical frame without distortion or shrinkage carried by histological slide, (2) 2D slice with arbitrary orientation, (3) digital format to facilitate data mapping, (4) probabilistic data to be more representative and (5) toolkits for surgery guidance and data analysis. The accuracy of labeling will be checked with cortical surface geometry and white matter fiber tractography as criteria. This digital package will be freely distributed to facilitate the neurobiological studies on primates. Diffusion tensor magnetic resonance microimaging will be used to set up the MRI atlas with high resolution and high contrasts. High contrasts and high resolution stem from diffusion tensor imaging and microimaging, respectively. Based on the digital atlas, pre-experiment surgery guidance toolkit and post-experiment data analysis toolkit will be developed and incorporated into a single user-friendly platform. Development of technologies and databases will be focused on through the four aims. First, high quality ex vivo and in vivo data will be acquired and the ex-in vivo data will be registered. Second, most of the gray and white matter neural structures will be labeled and segmented. Third, probabilistic atlas and labeling will be established by transforming the brain images to a template. Fourth, a platform for guidance of invasive procedure and analysis of the experimental data will be developed. The probabilistic atlas is the key and central part of the proposal. It provides the database in the digital package. Brain and head coordinates will be built up to standardize structure-location relationship system. With the atlas and coordinate systems, two toolkits provide the convenient and practical means for primate researchers to use the atlas. PUBLIC HEALTH RELEVANCE: The proposed three-dimensional digital probabilistic atlas of primates will provide an accurate, comprehensive and evolving digital account about the brain structure of primates. The toolkits developed to guide the surgery procedure and map the experimental data will bring precision, convenience and efficiency to facilitate the studies of primate brain function. And the understanding of human brain function will also be greatly enhanced because of the close relationship between primate brain and that of human.