Zeiss LSM710 Confocal Microscope for Shared Imaging Facility

描述（由申请人提供）：这是一份购买倒置蔡司LSM710共聚焦显微镜的资金申请，以增加5年的直立徕卡TCS SP2共聚焦显微镜，该显微镜将保留在同一共享成像设施中。这些仪器将由MIF- biotech管理，MIF是两个显微镜和成像设施（MIF）之一，其主要功能是满足中央校园共聚焦成像需求。现有的徕卡SP2仍然可以满足许多用户的需求，但另一种仪器是必要的。与全国许多机构一样，MIF的设施也看到了对活细胞和组织成像的需求日益增长，目前该系统已经超负荷，以至于MIF生物技术公司的工作人员需要实施复杂的调度规则，将使用时间限制在3小时以内，这对于想要进行延时成像和光漂白研究的研究人员来说是不够的。新仪器还将为这些研究提供新的能力，例如更好的光转换能力和更好地在舞台上维护组织的环境舞台。MIF-Biotech和拟议中的新共焦中心将搬进威尔大厅，这是一座耗资1.6亿美元的研究大楼，目前已接近完工。这座新建筑中的研究社区是高度跨学科和快速发展的；预计在未来几年内将雇用10-20名新教师，其中大多数将使用已经不堪重负的MIF-Biotech资源。该仪器将直接使11个居住在中央校园的主要用户受益，他们都是美国国立卫生研究院支持的研究经费的主要研究者。更广泛地说，它将为校园活细胞和组织成像提供新的资源，使研究人员能够更好地了解细胞信号传导、囊泡循环和分泌、细胞-基质相互作用以及细胞介导的发育和生殖过程的基本分子机制。公共卫生相关性：人类疾病往往是分子关联或信号事件出错的结果。所要求的仪器将使研究人员能够在显微镜下研究活细胞中的这些类型的分子机制。该仪器的主要用户进行的研究具有广泛的人类健康影响：生殖和发育，癌症生物学，软骨工程，糖尿病和脑功能。

DESCRIPTION (provided by applicant): This is a request for funds to purchase an inverted Zeiss LSM710 confocal microscope to augment a 5-year old upright Leica TCS SP2 confocal microscope that will remain in the same shared imaging facility. These instruments will be managed under MIF-Biotech, one of two Microscopy and Imaging Facilities (MIF), whose main function is to serve central campus confocal imaging needs. The existing Leica SP2 still functions adequately for many users, but another instrument is necessary. The MIF facility, like many across the country, are seeing an increased demand for live cell and tissue imaging, and currently the system is so overbooked that MIF-Biotech staff needed to implement complicated scheduling rules limiting usage to three hour blocks of time, which are inadequate for researchers wanting to carry out time lapse imaging and photobleaching studies. The new instrument will also enable new capabilities for these studies, such as better photoconversion capabilities and an environmental stage for better maintaining tissues on the stage. MIF-Biotech and the proposed new confocal will move into Weill hall, a $160-million research building now nearing completion. The research community in this new building is highly interdisciplinary and rapidly growing; 10-20 new faculty hires are expected in the next several years, the majority of whom will be using the already overburdened MIF-Biotech resources. This instrument will directly benefit 11 central-campus-residing major users, all of whom are principal investigators of NIH-supported research grants. More broadly it would provide new resources for live cell and tissue imaging on campus, enabling researchers to better understand the fundamental molecular mechanisms of cell signaling, vesicle recycling and secretion, cell-matrix interactions and cell-mediated developmental and reproductive processes. PUBLIC HEALTH RELEVANCE: Human diseases are often the result of molecular associations or signaling events that have gone wrong. The instrument requested will allow researchers to investigate these types of molecular mechanisms in live cells on the microscope stage. The major users of this instrument carry out research with implications to a broad spectrum of human health: reproduction and development, cancer biology, cartilage engineering, diabetes and brain function.