Ti:Sapphire Laser Upgrade to Zeiss LSM 510/Confocor 3 FCS System

描述(申请人提供)：约翰霍普金斯大学克里格艺术与科学学院、怀廷工程学院和医学院内由11名NIH资助的研究人员组成的多样化小组申请资金，以购买变色龙超II钛：蓝宝石多光子激光器；蔡司环境孵化器(用于调节温度、湿度和二氧化碳)；以及将新激光器集成到现有LSM 510可见光系统所需的NLO硬件，该系统配备了Confoor-3荧光相关光谱模块。由此产生的配置将显著升级Confoor-3的FCS能力；并增加目前Homewood园区所缺乏的新的活细胞/深层组织成像资源。这将是生物学、心理学、生物物理学、工程学以及最近在霍姆伍德扩大的神经科学存在的多个正在进行的研究项目的福音。该仪器位于约翰霍普金斯综合成像中心(IIC，http://www.jhu.edu/iic)，)，该中心是与纳米生物技术研究所(INBT，http://inbt.jhu.edu/index.php).)相关的核心设施IIC是霍姆伍德校园/霍普金斯大学范围内的显微镜资源，由克里格艺术与科学学院和怀廷工程学院联合支持(参见Lattman&Jones Letters，附录第2页)；并由多所大学、学校和系定期使用，包括&gt；80个实验室和250多个用户。我们的研究人员均由美国国立卫生研究院资助，致力于各种基础细胞生物学和生物医学工程相关问题的研究，包括但不限于：细胞分化和基因调控；疱疹病毒组装和成熟、膜运输、线粒体分裂、膜蛋白折叠、器官发生和昼夜节律神经元电路。在更新的配置中，LSM 510/Confoor 3将为我们不同的研究人员小组提供广泛的基础/高级体内和体外显微镜功能，这些功能目前在霍普金斯大学霍姆伍德园区是不存在的。新系统将纳入IIC现有的、完善的充值系统，以确保收回用于供应和维护的资金；它将通过我们方便的网络时间表免费提供给霍普金斯大学的所有感兴趣的用户；它将被纳入IIC的年度本科生/研究生课程。公共卫生相关性：钛：蓝宝石激光升级到LSM 510/Confoor-3荧光相关光谱系统将用于监测荧光生物分子和生物大分子复合体在天然亚细胞环境中的相互作用；以及用于深层组织和现场成像实验。

DESCRIPTION (provided by applicant): A diverse group of 11 NIH funded investigators, within the Johns Hopkins University Krieger School of Arts and Sciences, Whiting School of Engineering, and the School of Medicine request funds to purchase a Chameleon ULTRA II Ti:Sapphire multiphoton laser; a Zeiss environmental incubator (for modulating temperature, humidity, and CO2); and NLO hardware necessary to integrate the new laser onto an existing LSM 510 VIS system equipped with a Confocor-3 Fluorescence Correlation Spectroscopy module. The resulting configuration will significantly upgrade the FCS capability of the Confocor-3; and add a new live cell/deep tissue imaging resource that is presently absent from the Homewood campus. It will be a boon to multiple ongoing research programs within biology, psychology, biophysics, engineering, and the newly expanded neuroscience presence on Homewood. The instrument is situated in the Johns Hopkins Integrated Imaging Center (IIC, http://www.jhu.edu/iic), a core facility associated with the Institute of NanoBioTechnology (INBT, http://inbt.jhu.edu/index.php). The IIC is a Homewood campus/Hopkins-wide microscopy resource, jointly supported by the Krieger School of Arts and Sciences and the Whiting School of Engineering (see Lattman & Jones letters, page 2 of the appendix); and utilized regularly by multiple universities, schools, and departments comprising >80 laboratories and more than 250 users. Our investigators, all well funded through the NIH, work on a host of diverse basic cell biology and biomedical engineering related questions including, though not limited to: cell differentiation and gene regulation; Herpes virus assembly and maturation, membrane trafficking, mitochondrial fission, membrane protein folding, organogenesis, and circadian rhythm neuronal circuitry. In the updated configuration, the LSM 510/Confocor 3 will afford our disparate group of investigators a broad range of basic/advanced in vivo and in vitro microscopy capabilities that are presently absent from the Hopkins Homewood campus. The new system will be incorporated into the IIC's existing, well established recharge system to ensure recovery of funds for supplies and maintenance; it will be made freely available to all interested users Hopkins- wide through our convenient web-scheduler; and it will be incorporated into the IIC's annual undergraduate/graduate course offerings. PUBLIC HEALTH RELEVANCE: Ti:Sapphire Laser upgrade to LSM 510/Confocor-3 Fluorescence Correlation Spectroscopy System will be used for monitoring the interactions of fluorescent biomolecules and biomacromolecular complexes in their native sub-cellular environments; and for deep tissue and live imaging experiments.