Imaging and Computational Resources Core

对服务的描述 图像采集、图像处理、图像分析、荧光检测与解释 信号是所有项目中使用的基本工具。硬件和软件的可用性 完成这些任务，以及维护和提高现有能力的技术支持的可用性 而开发新的处理方法对于有效和全面地完成 建议进行的研究。 核心包括共聚焦显微镜、流式细胞术、原子力显微镜和总 内部反射荧光显微镜(TIRFM)，以及支持维护硬件以 用于视频图像处理和软件开发，以促进从 实验图像。此外，在当前的应用程序中，我们建议将核心功能扩展为包括 对计算资源的支持。该项目增加了两名合作调查员Micah Dembo 将提供分析牵引力显微镜实验的专业知识，而David 吉，罗切斯特理工学院的助理教授，在 罗切斯特大学生物医学工程教授，他是并行计算应用程序方面的专家。因此， 既有高端并行计算资源的能力，又有分析牵引显微镜的能力 实验是对Core提供的服务的重大补充。项目1、2、3和5是 从事将受益于这些新资源的研究方向，而项目4在较小程度上将 从这些功能的可用性中获益。在过去的授权期内，我们在迁移到 新的硬件平台，转向以数字成像为主的采集格式。其中一个主要目标是 对于核心在下一个资金时期将提供技术支持和资源，以改进 使这一新硬件成为可能，并促进其在拟议研究中的常规使用。我们也认识到 数字世界在不断发展，对已有技术的故障排除支持受到限制 在整个计划资助期间，将需要保持所需的成像能力水平。

DESCRIPTION OF SERVICES Image acquisition, image processing, image analysis, and detecfion and interpretafion of fiuorescence signals are fundamental tools used in all of the projects. The availability of hardware and software to accomplish these tasks, and the availability of technical support to maintain and improve exisfing capabilifies and to develop new processing methodologies is essential for the effective and fimely completion of the proposed studies. The core includes access to confocal microscopy, flow cytometry, atomic force microscopy and total internal refiectance fluorescence microscopy (TIRFM), as well as support for the maintenance of hardware to be used for video image processing and the development of software to facilitate extraction of information from experimental images. In addifion, in the current application we propose to expand core capabilifies to include support for computational resources. Two Co-lnvestigators have been added to the project, Micah Dembo from Boston University, will provide expertise in analyzing traction force microscopy experiments, and David Gee, an Assistant Professor at Rochester Institute of Technology who holds an adjunct appointment in Biomedical Engineering at the University of Rochester, who is expert in parallel compufing applications. Thus, capabilities for both high end parallel compufing resources and the ability to analyze traction force microscopy experiments represent significant addifions to services provided by the Core. Projects 1, 2, 3 and 5 are engaged in research directions that will benefit from these new resources, and Project 4, to a lesser extent, will benefit from the availability of these capabilities. We have made progress in the past grant period in moving to new hardware platforms, moving to a largely digital imaging acquisition format. One of the primary objectives for the Core in the next funding period will be to provide the technical support and resources for improving capabilifies of this new hardware and facilitafing its roufine use in the proposed studies. We also recognize that the digital worid is constantly evolving, and confinued support to troubleshoot established technologies and maintain needed levels of imaging capability will be needed throughout the funded period of the program.