Research Resource for Integrated Glycotechnology

复合碳水化合物使细胞表面精致，有助于细胞外基质，以及 装饰排泄出来的糖蛋白。在这里，它们调节着对正常生活至关重要的各种各样的过程 生命过程的执行。它们也进入异常的过程，如病原体的入侵和 正常细胞的致癌转化。了解碳水化合物结构的多样性，分子 它的起源基础和识别它的分子基础对诊断和治疗非常重要 疾病的治疗。在这份提案中，我们概述了集成糖基技术的研究资源，它将 通过开发研究碳水化合物-蛋白质相互作用的新方法来服务于社区 分子水平，并通过协作、服务和培训活动传播这些方法。 在分子水平上描述碳水化合物与蛋白质的相互作用并非易事。 碳水化合物的结构本身是非常多样化的。此外，了解它们的交互，并使用 对干预疾病过程的理解，不仅需要关于碳水化合物的信息，而且还需要关于 决定它们的合成和识别的蛋白质。解决如此重大的特征问题 而复杂性需要一套基于广泛的分析、生化、化学和 计算科学。这些工具在集成在一起时最有用，可以产生高效和多功能的 可以回答发现新的碳水化合物-蛋白质相互作用系统所引起的问题的资源。 拟议的努力包括开发生产适量蛋白质的技术。 为了进行物理和生化表征，介绍碳水化合物的合成方法 由于有足够的多样性和纯度来探索亲和力的分子基础，新工具的开发 碳水化合物-蛋白质相互作用的质谱学和核磁学结构表征 共振，并开发了一个计算平台，将数据整合成一个完整的 蛋白质-碳水化合物相互作用的分子图像。

Complex carbohydrates elaborate the surfaces of cells, contribute to the extracellular matrix, and decorate excreted glycoproteins. Here they mediate a wide variety of processes that are essential to the normal execution of life processes. They also enter into abnormal processes, such as the invasion of pathogens and the oncogenic transformation of normal cells. Understanding the diversity of carbohydrate structure, the molecular basis for its origin, and the molecular basis for its recognition are extremely important to the diagnosis and treatment of disease. In this proposal we outline a Research Resource for Integrated Glycotechnology that will serve the community through the development of new approaches to study carbohydrate-protein interactions at a molecular level and the dissemination of these approaches through collaborative, service, and training activities. Characterization of carbohydrate-protein interactions at a molecular level is not an easy task. Carbohydrate structures are in themselves very diverse. Moreover, understanding their interactions, and using that understanding to intervene in disease processes, requires information on not only the carbohydrates, but also the proteins that dictate their synthesis and recognition. Tackling a characterization problem of this magnitude and complexity requires a diverse set of tools based on a wide range of analytical, biochemical, chemical, and computational science. These tools are most useful when integrated to produce an efficient and versatile resource that can answer questions that arise with the discovery of new carbohydrate-protein interacting systems. Proposed efforts include development of techniques for the production of proteins in adequate amounts to allow physical and biochemical characterization, introduction of methods for the synthesis of carbohydrates with sufficient diversity and purity to probe the molecular basis of affinity, the development of new tools for the structural characterization of the carbohydrate-protein interactions by mass spectrometry and nuclear magnetic resonance, and the development of a computational platform for the integration of data into a complete molecular picture of protein-carbohydrate interactions.