A Proteomics Research Resource for Integrative Biology

描述(总体)：能够精确和准确地测量几乎所有表达的蛋白质及其修饰形式的水平，可以为细胞的分子性质和操作、细胞信号通路和网络、细胞周期、细胞分化和其他与人类健康和各种疾病状态的进展相关的过程提供新的见解。该资源中心致力于通过开发和整合用于生物应用的新蛋白质组技术，传播新技术，并培训科学家使用这些技术，为NIH支持的生物医学研究社区提供服务。在最初的4年里，资源中心培养了一支强大的研究团队，他们取得了良好的出版记录，开展了一系列富有成效的合作项目，并有效地传播了一系列技术和信息学的发展。因此，该中心现在正准备取得更大的成功。资源中心核心的主要技术目标是开发和应用一套集成的生物学方法、新的分析技术以及相关的计算和信息学工具，用于比目前可能的更快速、更定量、更敏感和更全面的蛋白质组学测量应用。资源中心的三个技术核心旨在发展：i)改进的空间和时间蛋白质组学技术，其中包括更好地确定蛋白质的空间定位的方法，主要是关于膜的空间定位和量化蛋白质之间的动力学；2)对蛋白质组进行更高的吞吐量、更灵敏和更稳健的定量测量，以提供更大的动态范围和更大的蛋白质及其修饰的覆盖面；以及3)需要计算和生物信息学工具来提供基于数据的信息和统计上合理的质量测量，以促进新的生物学理解。中心的技术发展将在一系列具有挑战性的合作应用的背景下进行评估，这些应用是根据科学和生物医学的相关性以及它们从资源中心的能力中受益的能力而选择的。应用范围包括微生物致病性、创伤研究、细胞迁移、肾移植、信号转导、神经蛋白质组学、辐射反应、慢性毒力感染、生物标记物发现和乳腺肿瘤学。

DESCRIPTION (Overall): The ability to precisely and accurately measure levels of nearly all expressed proteins and their modified forms can provide new insights into the molecular nature and operation of cells, cell signaling pathways and networks, the cell cycle, cellular differentiation, and other processes relevant to human health and to the progression of various disease states. This Resource Center is focused on serving the NIH-supported biomedical research community by developing and integrating new proteomic technologies for biological applications, disseminating the new technologies, and training scientists in their use. In its first 4 years the Resource Center has cultivated a strong team of researchers, who have achieved a strong publication record, a productive set of collaborative projects, and provided effective dissemination of an array of technology and informatics developments. As a result the Center is now poised for even greater successes. The primary technology objectives of the Resource Center Cores are to develop and apply an integrated set of biological methods, new analytical technologies, and associated computational and informatics tools, for much more rapid, quantitative, sensitive, and comprehensive proteomics measurement applications than presently possible. The three technological Cores of the Resource Center aim at development of: i) improved technologies for spatial and temporal proteomics, which includes approaches to better define the compartment localization of proteins, primarily with respect to membrane compartments and to quantify the dynamics of proteins between compartments; 2) higher throughput, more sensitive, and much more robust quantitative measurements of proteomes that provide broader dynamic range and greater coverage of proteins and their modifications; and 3) computational and bioinformatics tools needed to provide information based on data with statistically sound measures of quality, so as to facilitate new biological understandings. The Center's technology developments will be evaluated in the context of a range of challenging collaborative applications selected on the basis of their scientific and biomedical relevance, as well as their capacity to benefit from the Resource Center's capabilities. The scope of applications include areas of microbial pathogenicity, trauma research, cellular migration, kidney transplantation, signal transduction, neuroproteomics, radiation response, chronic virulent infections, biomarker discovery, and mammary oncology.